Stetler G, Brewer M T, Thompson R C
Nucleic Acids Res. 1986 Oct 24;14(20):7883-96. doi: 10.1093/nar/14.20.7883.
We report the isolation of the human gene encoding an inhibitor of neutrophil elastase and cathepsin G. We have sequenced the gene and a cDNA clone isolated from human parotid tissue. The protein encoded by this gene appears to contain two functional domains, one having a trypsin inhibitory site and the other an elastase inhibitory site. The two-domain structure of the protein is reflected in the organization of the gene, with each domain represented by a separate exon. We have also noted that the intervening sequence separating the trypsin-inhibitor-exon and the elastase-inhibitor-exon is flanked by eleven base-pair direct repeats, suggesting that this intron may have been generated by a transposition-type event.
我们报告了编码中性粒细胞弹性蛋白酶和组织蛋白酶G抑制剂的人类基因的分离。我们已对该基因以及从人腮腺组织中分离出的一个cDNA克隆进行了测序。该基因编码的蛋白质似乎包含两个功能结构域，一个具有胰蛋白酶抑制位点，另一个具有弹性蛋白酶抑制位点。蛋白质的双结构域结构反映在基因的组织中，每个结构域由一个单独的外显子代表。我们还注意到，分隔胰蛋白酶抑制剂外显子和弹性蛋白酶抑制剂外显子的间隔序列两侧有11个碱基对的直接重复序列，这表明该内含子可能是由转座型事件产生的。
