São Paulo State University "Júlio de Mesquita Filho" (UNESP), Institute of Biosciences, Department of General and Applied Biology, 24A Ave, 1515, Bela Vista, Rio Claro, SP, 13506-700, Brazil.
Embrapa Southeast Livestock, Rodovia Washington Luiz, km 234, Fazenda Canchim, São Carlos, SP, 13560-970, Brazil.
Exp Parasitol. 2023 Jan;244:108423. doi: 10.1016/j.exppara.2022.108423. Epub 2022 Nov 17.
The study analyzed the chemical composition and the acaricide effect of Egletes viscosa Less (macela-da-terra) and Lippia schaueriana Mart. (lipia-da-serra) essential oils (EOs) on Rhipicephalus sanguineus s. l. (Acari: Ixodidae) engorged females. The chemical analysis (GC-MS and GC-FID) identified 27 components in E. viscosa EO and 18 in L. schaueriana EO, which comprise more than 98% of its constituents. The effects of the oils on the reproductive biology of R. sanguineus ticks were assessed by adult immersion test. Both EOs significantly reduced (p < 0.05) the egg production index when the females were exposed to 25 and 50 mg/mL, also affecting the egg viability. During the laying process, the eggs produced by the females exposed to the EO showed several morphological alterations such as dehydrated, darkened, and disaggregated, and these alterations were more severe as the concentrations increased. The mortality percentages were 58.9%, 70.8% and 92.7% when the ticks were exposed to 12.5, 25 and 50 mg/mL of E. viscosa oil, respectively. In the same concentrations, the efficacy of L. schaueriana was 39.3%, 53.4%, and 84.6%. Therefore, it can be concluded that the essential oils of E. viscosa and L. schaueriana have acaricidal effect in females of R. sanguineus s.l ticks.
本研究分析了 Egletes viscosa Less（macela-da-terra）和 Lippia schaueriana Mart.（lipia-da-serra）精油（EO）的化学成分和杀螨效果对 Rhipicephalus sanguineus s. l.（蜱螨目：硬蜱科）饱血雌蜱的影响。化学分析（GC-MS 和 GC-FID）鉴定出 E. viscosa EO 中有 27 种成分，L. schaueriana EO 中有 18 种成分，它们占其组成的 98%以上。通过成虫浸浴试验评估了这些油对 R. sanguineus 蜱生殖生物学的影响。当雌蜱暴露于 25 和 50 mg/mL 时，两种 EO 均显著降低（p<0.05）产卵指数，也影响卵的活力。在产卵过程中，暴露于 EO 的雌蜱所产的卵出现多种形态改变，如脱水、变暗和分解，随着浓度的增加，这些改变更加严重。当蜱暴露于 12.5、25 和 50 mg/mL 的 E. viscosa 油时，死亡率分别为 58.9%、70.8%和 92.7%。在相同浓度下，L. schaueriana 的功效为 39.3%、53.4%和 84.6%。因此，可以得出结论，E. viscosa 和 L. schaueriana 的精油对 R. sanguineus s.l. 雌蜱具有杀螨作用。
