School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, PR China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou, 310012, PR China; Instrumental Analysis Center of Zhejiang Gongshang University, Hangzhou, 310012, PR China; Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Hangzhou, 310012, PR China.
School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, PR China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou, 310012, PR China.
Environ Pollut. 2023 Jan 1;316(Pt 1):120666. doi: 10.1016/j.envpol.2022.120666. Epub 2022 Nov 17.
Although the environmental and health risks of chlorinated organophosphate esters (OPEs-Cl) have drawn much attention, its environmental behaviors have been insufficiently characterized. As a notable sink of this emerging contaminant, non-sanitary landfills, which may decompose/accumulate OPEs-Cl, is of particular concern. In the present study, the dynamic processes of the typical OPEs-Cl, tris(2-chloroethyl) phosphate (TCEP), in non-sanitary landfill soils were analyzed under anaerobic condition, and the microbial taxa involved in these processes were explored. Our results showed that TCEP could be simultaneously reduced by abiotic and biotic processes, as it was reduced by 73.9% and 65.5% over the 120-day experiment in landfill humus and subsoil, respectively. Notably, the degradation of TCEP was significantly (p < 0.05) enhanced under the stress of a high TCEP concentration (10 μg g), while its ecological consequences were found insignificant regarding the microbial diversity and community structure and the typical soil redox processes, including Fe(III)/SO reduction and methanogenesis, in both soils. The microbial diversity of subsoil was significantly lower, and acetate was an important factor in changing microbial communities in landfill soils. The microbes in the family Nocardioidaceae and genus Pseudomonas might contribute to in the degradation of TCEP in landfill humus and subsoil, respectively. The metabolism related to sulfur and sulfate respiration were significantly (p < 0.05) correlated with TCEP reduction, and Desulfosporosinus were found as a potentially functional microbial taxon in TCEP degradation in both soils. The results could advance our understanding of the environmental behavior of OPEs-Cl in landfill-like complex environments.
虽然含氯有机磷酸酯（OPEs-Cl）的环境和健康风险引起了广泛关注，但它们的环境行为尚未得到充分描述。作为这种新兴污染物的一个显著汇，可能会分解/积累 OPEs-Cl 的非卫生垃圾填埋场尤其值得关注。在本研究中，分析了典型 OPEs-Cl（三（2-氯乙基）磷酸酯（TCEP））在厌氧条件下在非卫生垃圾填埋场土壤中的动态过程，并探讨了参与这些过程的微生物类群。结果表明，TCEP 可以通过非生物和生物过程同时还原，在填埋腐殖质和底土中，120 天实验中 TCEP 分别还原了 73.9%和 65.5%。值得注意的是，在高 TCEP 浓度（10μg g）的胁迫下，TCEP 的降解显著增强（p < 0.05），而其生态后果在两种土壤的微生物多样性和群落结构以及典型的土壤氧化还原过程（包括 Fe（III）/SO 还原和产甲烷作用）中并不明显。底土的微生物多样性明显较低，乙酸盐是改变填埋场土壤微生物群落的重要因素。诺卡氏菌科和假单胞菌属的微生物可能分别有助于填埋腐殖质和底土中 TCEP 的降解。与硫和硫酸盐呼吸相关的代谢与 TCEP 还原显著相关（p < 0.05），并发现脱硫螺旋菌是两种土壤中 TCEP 降解的潜在功能微生物类群。研究结果可以提高我们对 OPEs-Cl 在类似垃圾填埋场的复杂环境中的环境行为的认识。
