Knockdown of Stromal Interaction Molecule 1 () Suppresses Acute Myeloblastic Leukemia-M5 Cell Line Survival Through Inhibition of Reactive Oxygen Species Activities.

OBJECTIVE

This study aimed to investigate the role of the stromal interaction molecule 1 () gene in the survival of the acute myeloblastic leukemia (AML)-M5 cell line (THP-1).

MATERIALS AND METHODS

The effect was assessed via dicersubstrate siRNA-mediated knockdown. The effect of knockdown on the expression of and pathway-related genes and reactive oxygen species (ROS) generation-related genes was tested using real-time polymerase chain reaction. Cellular functions, including ROS generation, cell proliferation, and colony formation, were also evaluated following knockdown.

RESULTS

The findings revealed that knockdown reduced intracellular ROS levels via downregulation of and . These findings were associated with the downregulation of , and was also downregulated, while was upregulated following knockdown. Furthermore, knockdown reduced THP-1 cell proliferation and colony formation.

CONCLUSION

This study has demonstrated the role of in promoting AML cell proliferation and survival through enhanced ROS generation and regulation of AKT/MAPK-related pathways. These findings may help establish as a potential therapeutic target for AML treatment.