Ay Arzu, Alkanli Nevra, Kurt Idris, Ustundag Sedat, Sipahi Tammam, Sut Necdet
Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey.
Department of Biophysics, Faculty of Medicine, Haliç University, Istanbul, Turkey.
J Diabetes Metab Disord. 2022 Jun 7;21(2):1317-1326. doi: 10.1007/s40200-022-01061-9. eCollection 2022 Dec.
The goal of this study is to determine the role of MTHFR (C677T, A1298C) and MGP (G-7A, T-138C) gene variations in DN development.
There were 61 DN patients and 55 healthy controls in this study. The genotype distributions of these gene variations were determined using PCR and RFLP methods.
According to our study, there was no significant relationship between these gene variations and DN ( > 0.05). The allele frequencies of the MTHFR C677T gene variation in the control group were found significantly different from the Hardy-Weinberg distribution ( < 0.05). According to combined genotype analysis, GA-TT combined genotype of MGP (G-7A/T-138C) gene variations was observed significantly more in the patient group with DN. The GA-TT combined genotype of MGP (G-7A/T-138C) gene variations was differ significantly between these groups (OR: 2.359, %95 CI: 1.094-5.087, = 0.028).
In our study, although MTHFR and MGP gene variations were not risk factors, the GA-TT combined genotype of the MGP (G-7A/T-138C) gene variation was detected as an important risk factor for DN.
本研究的目的是确定亚甲基四氢叶酸还原酶（MTHFR，C677T、A1298C）和基质Gla蛋白（MGP，G-7A、T-138C）基因变异在糖尿病肾病（DN）发生发展中的作用。
本研究纳入61例DN患者和55例健康对照者。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）方法确定这些基因变异的基因型分布。
根据我们的研究，这些基因变异与DN之间无显著相关性（P＞0.05）。发现对照组中亚甲基四氢叶酸还原酶C677T基因变异的等位基因频率与哈迪-温伯格分布有显著差异（P＜0.05）。根据联合基因型分析，在DN患者组中，MGP（G-7A/T-138C）基因变异的GA-TT联合基因型显著更多见。MGP（G-7A/T-138C）基因变异的GA-TT联合基因型在这些组之间有显著差异（比值比：2.359，95%可信区间：1.094 - 5.087，P = 0.028）。
在我们的研究中，虽然MTHFR和MGP基因变异不是危险因素，但MGP（G-7A/T-138C）基因变异的GA-TT联合基因型被检测为DN的一个重要危险因素。
