Pirozzi Flavio Fontes, Belini Junior Edis, Okumura Jessika Viviani, Salvarani Mariana, Bonini-Domingos Claudia Regina, Ruiz Milton Artur
Departamento de Biologia, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brasil.
Arch Endocrinol Metab. 2018 Feb;62(1):21-26. doi: 10.20945/2359-3997000000005.
Objectives This study aimed to evaluate the frequencies of the angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) and methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphisms in obese patients with and without type 2 diabetes mellitus (T2DM). Subjects and methods These polymorphisms were analyzed by polymerase chain reaction in 125 patients with obesity, 47 (T2DM) and 78 (Control Group). Results No significant difference was found on comparing the T2DM and Control Groups in respect to the genotypic frequencies of the polymorphisms - (II: 13.3% vs. 12.0%; ID: 37.8% vs. 37.3; DD: 48.9% vs. 50.7%; CC: 36.2% vs. 39.0%; CT: 46.8% vs. 49.3%; TT: 17.0% vs. 11.7%), and alleles (I: 32.2% vs. 30.7%; D: 67.8% vs. 69.3%; C: 59.6% vs. 63.6%; T: 40.4% vs. 36.4%) and their synergisms in the pathophysiology of T2DM. On analyzing the T2DM Group, there were no significant differences in the presence of complications. In this population of Brazilian obese patients, no correlation was found between the ACE and MTHFR polymorphisms in the development of T2DM. Conclusion Analyzing only the group with diabetes, there was also no relationship between these polymorphisms and comorbidities.
目的 本研究旨在评估血管紧张素转换酶（ACE）基因插入/缺失（I/D）和亚甲基四氢叶酸还原酶（MTHFR）基因C677T多态性在伴有和不伴有2型糖尿病（T2DM）的肥胖患者中的频率。
受试者与方法 通过聚合酶链反应对125例肥胖患者（47例T2DM患者和78例对照组）的这些多态性进行分析。
结果 在比较T2DM组和对照组的多态性基因型频率时未发现显著差异 - （II：13.3% 对 12.0%；ID：37.8% 对 37.3%；DD：48.9% 对 50.7%；CC：36.2% 对 39.0%；CT：46.8% 对 49.3%；TT：17.0% 对 11.7%），以及等位基因（I：32.2% 对 30.7%；D：67.8% 对 69.3%；C：59.6% 对 63.6%；T：40.4% 对 36.4%）及其在T2DM病理生理学中的协同作用方面。在分析T2DM组时，并发症的存在没有显著差异。在这组巴西肥胖患者中，未发现ACE和MTHFR多态性与T2DM发生之间存在相关性。
结论 仅分析糖尿病组时，这些多态性与合并症之间也没有关系。
