Nandi Abhishek, Yadav Ruchi, Singh Aditi
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow, India.
Front Med (Lausanne). 2022 Nov 4;9:1047752. doi: 10.3389/fmed.2022.1047752. eCollection 2022.
is a bacterial pathogen that is commonly associated with hospital-acquired illnesses. Antimicrobial drug resistance in includes several penicillin classes, first and second-generation cephalosporins, cephamycins, most aminoglycosides, chloramphenicol, and tetracyclines. The recent rise in multidrug-resistant strains has resulted in an increase in pneumoniae associated with ventilators, urinary tract infections associated with the catheter, and bloodstream infections, all of which have increased complications in treatment, cost of treatment, and death. Small compounds known as antimicrobial peptides (AMPs) are known to have damaging effects on pathogenic bacteria. To determine their antimicrobial activity, AMPs are created from proteins acquired from various sources and evaluated . In the last phase of lytic cycle, bacteriophages release hydrolytic enzymes called endolysins that cleave the host's cell wall. Due to their superior potency and specificity compared to antibiotics, lysins are used as antibacterial agents. In the present study, different types of endolysin from phages of were selected based on an extensive literature survey. From the PhaLP database, the sequences of the selected lysins were retrieved in FASTA format and antimicrobial peptides were found among them. With the help of available bioinformatic tools, the anti-biofilm property, anti-fungal property, cell-penetrating property, and cellular toxicity of the antimicrobial peptides were determined. Out of the fourteen antimicrobial peptides found from the eight selected endolysins of specific phage, eight of them has anti-biofilm property, nine of them has anti-fungal property, five of them has cell-penetrating property and all of them are non-toxic.
是一种通常与医院获得性疾病相关的细菌病原体。对多种抗菌药物耐药，包括几种青霉素类、第一代和第二代头孢菌素、头霉素、大多数氨基糖苷类、氯霉素和四环素。近年来多重耐药菌株的增加导致与呼吸机相关的肺炎、与导管相关的尿路感染和血流感染增多，所有这些都增加了治疗并发症、治疗成本和死亡率。已知称为抗菌肽（AMPs）的小分子化合物对病原菌有破坏作用。为了确定其抗菌活性，从各种来源获得的蛋白质中产生抗菌肽并进行评估。在裂解周期的最后阶段，噬菌体释放称为内溶素的水解酶，这些酶可裂解宿主细胞壁。由于与抗生素相比具有更高的效力和特异性，溶素被用作抗菌剂。在本研究中，基于广泛的文献调查，从噬菌体中选择了不同类型的内溶素。从PhaLP数据库中，以FASTA格式检索所选溶素的序列，并在其中发现抗菌肽。借助现有的生物信息学工具，确定了抗菌肽的抗生物膜特性、抗真菌特性、细胞穿透特性和细胞毒性。从特定噬菌体的八个选定内溶素中发现的十四种抗菌肽中，其中八种具有抗生物膜特性，九种具有抗真菌特性，五种具有细胞穿透特性，并且所有这些都无毒。
