Li Guangquan, Wang Xianze, Liu Yi, Wang Cui, Yang Yunzhou, Gong Shaoming, Zhu Lihui, He Daqian, Wang Huiying
Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai, China.
Front Vet Sci. 2022 Nov 3;9:1006318. doi: 10.3389/fvets.2022.1006318. eCollection 2022.
The study aimed to investigate the effects of honeysuckle extract (HE) on growth performance, serum biochemical indexes, immune organ indexes, gut morphology, and gut microbes in geese. A total of 180 28-day-old Holdobaki geese were randomly divided into three groups. Each group contained 6 replicates (10 geese, with 5 males and 5 females). The BD group was fed the basal diet, the HE1 group was fed the basal diet supplemented with 1 g/kg of HE, and the HE2 group was fed the basal diet supplemented with 2 g/kg of HE. The experiment lasted for 42 days. The results showed that, compared with the BD group, the average daily gain (ADG) of the HE1 and HE2 groups tended to increase (0.05 < < 0.10), but the average daily feed intake (ADFI) and final body weight (BW) did not differ significantly, and the feed/gain ratio (F/G) was significantly lower ( < 0.01). The bursa index and the thymus index tended to increase (0.05 < < 0.10), and serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels increased significantly ( < 0.05). In the HE1 and HE2 groups, the crypt depth (CD) in the jejunum tended to decrease (0.05 < < 0.10), and the villus height/crypt depth ratio (V/C) increased significantly in the jejunum and the ileum ( < 0.05). According to 16sRNA microbial community diversity analysis, , and were the dominant phyla. The abundance of was significantly decreased ( < 0.01), while that of was significantly increased ( < 0.01), in the HE1 and HE2 groups compared with the BD group. , and were the dominant species, and the abundance of and was significantly increased ( < 0.05). According to the LEfSe analysis, BD enriched and ; HE1 enriched g_, and ; and HE2 enriched g_, and . According to the Spearman correlation analysis, was positively correlated with final BW, ADG, IgA, IgG, VH (ileum), and V/C (ileum) and was negatively correlated with F/G and CD (ileum); was negatively correlated with final BW, ADG, IgA, and IgG. HE supplementation at 1 g/kg improved growth performance, immune performance, gut morphology, and cecal microbes.
本研究旨在探讨金银花提取物（HE）对鹅生长性能、血清生化指标、免疫器官指数、肠道形态及肠道微生物的影响。选取180只28日龄的霍尔多巴基鹅，随机分为三组。每组设6个重复（每组10只鹅，公母各5只）。BD组饲喂基础日粮，HE1组饲喂添加1 g/kg HE的基础日粮，HE2组饲喂添加2 g/kg HE的基础日粮。试验持续42天。结果表明，与BD组相比，HE1组和HE2组的平均日增重（ADG）有增加趋势（0.05＜ ＜0.10），但平均日采食量（ADFI）和末重（BW）差异不显著，料重比（F/G）显著降低（ ＜0.01）。法氏囊指数和胸腺指数有增加趋势（0.05＜ ＜0.10），血清免疫球蛋白A（IgA）和免疫球蛋白G（IgG）水平显著升高（ ＜0.05）。在HE1组和HE2组中，空肠隐窝深度（CD）有降低趋势（0.05＜ ＜0.10），空肠和回肠绒毛高度/隐窝深度比值（V/C）显著升高（ ＜0.05）。根据16sRNA微生物群落多样性分析， 、 和 为优势菌门。与BD组相比，HE1组和HE2组中 的丰度显著降低（ ＜0.01），而 的丰度显著增加（ ＜0.01）。 、 和 为优势菌种， 和 的丰度显著增加（ ＜0.05）。根据LEfSe分析，BD组富集 和 ；HE1组富集g_和 ；HE2组富集g_和 。根据Spearman相关性分析， 与末重、ADG、IgA、IgG、回肠绒毛高度（VH）和回肠V/C呈正相关，与F/G和回肠CD呈负相关； 与末重、ADG、IgA和IgG呈负相关。添加1 g/kg的HE可改善鹅的生长性能、免疫性能、肠道形态和盲肠微生物。
