Vos Marten H, Salman Mayla, Liebl Ursula
Laboratoire d'Optique et Biosciences, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, École Polytechnique, Institut Polytechnique de Paris, Paris, France.
Front Mol Biosci. 2022 Nov 3;9:1046412. doi: 10.3389/fmolb.2022.1046412. eCollection 2022.
Carbon monoxide has been recognized relatively recently as signaling molecule, and only very few dedicated natural CO sensor proteins have been identified so far. These include in particular heme-based transcription factors: the bacterial sensor proteins CooA and RcoM. In these 6-coordinated systems, exchange between an internal protein residue and CO as a heme ligand in the sensor domain affects the properties of the DNA-binding domain. Using light to dissociate heme-ligand bonds can in principle initiate this switching process. We review the efforts to use this method to investigate early processes in ligand switching and signaling, with an emphasis on the CO-"trappingˮ properties of the heme cavity. These features are unusual for most heme proteins, but common for heme-based CO sensors.
一氧化碳直到最近才被公认为一种信号分子，到目前为止仅鉴定出极少数专门的天然CO传感蛋白。其中特别包括基于血红素的转录因子：细菌传感蛋白CooA和RcoM。在这些六配位系统中，传感器结构域中内部蛋白质残基与作为血红素配体的CO之间的交换会影响DNA结合结构域的特性。原则上，利用光解离血红素-配体键可以启动这种转换过程。我们综述了利用该方法研究配体转换和信号传导早期过程的相关工作，重点关注血红素腔的CO“捕获”特性。这些特征对于大多数血红素蛋白来说并不常见，但对于基于血红素的CO传感器来说却很常见。
