Zhu Jiajia, Chen Yunsheng, Wu Yifan, Wang Yongqiang, Zhu Kui
National Center for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
Front Microbiol. 2022 Nov 4;13:1010584. doi: 10.3389/fmicb.2022.1010584. eCollection 2022.
-associated infectious coryza (IC) is an important threat in commercial poultry. Previous studies about the characteristics of are succeeded in revealing the course of IC disease, but whether and how resident microbes contribute to the infection remains unclear. To understand the role of commensal bacteria, we isolated 467 commensal bacteria, including 38 , from the respiratory tract of IC chicken. The predominant commensal isolates were Gram-positive bacteria belonging to spp. [33.19%, 95% confidence interval (CI): 28.93-37.66%], spp. (16.49%, 95% CI: 13.23-20.17%), and spp. (16.27%, 95% CI: 13.04-19.94%). These isolates were closely correlated with the survival of . We examined and found that commensal bacteria aggravate -associated infections because certain commensal species (28.57%, 95% CI: 15.72-44.58%) induced hemolysis and promoted the growth of . Notably, showed high resistance to routine antibiotics such as erythromycin (84.21%, 95% CI: 68.75-93.98%), tetracycline (73.68%, 95% CI: 56.90-86.60%) and carried diverse mobile resistance gene clusters. Overall, we found commensal bacteria especially Gram-positive bacteria facilitate the survival of multidrug-resistant to exacerbate infections, suggesting that novel strategies may diminish -associated infections by modulating the population dynamics of commensal bacteria.
相关传染性鼻炎（IC）是商业家禽业面临的重要威胁。以往关于[具体研究对象]特征的研究成功揭示了IC疾病的病程，但常驻微生物是否以及如何促成感染仍不清楚。为了解共生细菌的作用，我们从IC病鸡的呼吸道中分离出467种共生细菌，其中包括38种[具体细菌种类]。主要的共生分离菌是革兰氏阳性菌，属于[具体菌属] spp.（33.19%，95%置信区间（CI）：28.93 - 37.66%）、[具体菌属] spp.（16.49%，95% CI：13.23 - 20.17%）和[具体菌属] spp.（16.27%，95% CI：13.04 - 19.94%）。这些分离菌与[具体病原体]的存活密切相关。我们研究发现共生细菌会加剧[具体病原体]相关感染，因为某些共生菌种（28.57%，95% CI：15.72 - 44.58%）会诱导溶血并促进[具体病原体]的生长。值得注意的是，[具体病原体]对红霉素（84.21%，95% CI：68.75 - 93.98%）、四环素（73.68%，95% CI：56.90 - 86.60%）等常规抗生素表现出高度耐药性，并携带多种移动耐药基因簇。总体而言，我们发现共生细菌尤其是革兰氏阳性菌有助于多重耐药[具体病原体]的存活，从而加剧感染，这表明新的策略可能通过调节共生细菌的种群动态来减少[具体病原体]相关感染。
