Wahyuni Agnesia Endang Tri Hastuti, Tabbu Charles Rangga, Artanto Sidna, Setiawan Dwi Cahyo Budi, Rajaguguk Sadung Itha
Department of Microbiology, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta, Indonesia.
Department of Pathology, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta, Indonesia.
Vet World. 2018 Apr;11(4):519-524. doi: 10.14202/vetworld.2018.519-524. Epub 2018 Apr 23.
Infectious coryza (IC) or snot is an infectious upper respiratory disease affecting chickens and birds, including quails, and it is caused by . The symptoms of IC are facial swelling, malodorous nasal discharge, and lacrimation. This study aimed to isolate, identify, and serotype the of snot in quails and to determine the sensitivity and resistance to several antibiotics.
Nine quails from Yogyakarta, Indonesia with typical snot disease symptoms were used in this study. The nasal swab was obtained and directly streaked onto a chocolate agar plate and blood agar plate (BAP), then incubated in 5% CO at 37°C for 24-48 h. spp. was cross-streaked onto the BAP to show the satellite growth. The observation of the morphology of the suspected colony, Gram staining, and biochemical tests (catalase test, oxidase test, urease test, peptone test, and carbohydrate fermentation such as maltose, mannitol, lactose, and sorbitol) are done to identify the species of bacteria. This research also detects the serovar of using hemagglutination inhibition test.The antibiotic sensitivity tests were also performed using several antibiotics against five isolates that were cultured on Mueller-Hinton Agar and added with antibiotic discs, then incubated in 5% CO at 37°C for 24-48 h.
Five isolates out of nine suspected isolates (55.5%) were . The growth of isolates from quails did not depend on the nicotinamide adenine dinucleotide (NAD) (NAD-independent). Sensitivity test was done using the five identified isolates, results showed that they were 100% sensitive to amoxicillin (AMC) and ampicillin (AMP); 100% resistant toward amikacin (AK), erythromycin (E), gentamycin (CN), and tetracycline (TE); 80% resistant toward kanamycin (K) and trimethoprim (W); 60% resistant toward chloramphenicol (C); and 20% toward enrofloxacin (ENR). The antibiotics that have an intermediate sensitivity (in between sensitive and resistant) were ENR and K, 80% and 20%, respectively. Three out of five isolates were identified as serovar B of using HI test.
Five out of nine isolates (55.5%) from quails with typical IC disease symptoms identified as and sensitive toward AMC and AMP. Three out of five isolates were identified as serovar B.
传染性鼻炎（IC）或鼻涕病是一种影响鸡和鸟类（包括鹌鹑）的传染性上呼吸道疾病，由……引起。IC的症状包括面部肿胀、恶臭的鼻分泌物和流泪。本研究旨在分离、鉴定鹌鹑鼻涕病的……并进行血清分型，以及确定其对几种抗生素的敏感性和耐药性。
本研究使用了来自印度尼西亚日惹的9只具有典型鼻涕病症状的鹌鹑。采集鼻拭子并直接划线接种于巧克力琼脂平板和血琼脂平板（BAP）上，然后在5%二氧化碳环境中于37°C孵育24 - 48小时。……菌在BAP上进行交叉划线以显示卫星生长。对疑似菌落的形态、革兰氏染色以及生化试验（过氧化氢酶试验、氧化酶试验、脲酶试验、蛋白胨试验以及麦芽糖、甘露醇、乳糖和山梨醇等碳水化合物发酵试验）进行观察以鉴定细菌种类。本研究还使用血凝抑制试验检测……的血清型。还使用几种抗生素对在Mueller - Hinton琼脂上培养并添加抗生素纸片的5株……分离株进行抗生素敏感性试验，然后在5%二氧化碳环境中于37°C孵育24 - 48小时。
9株疑似分离株中有5株（55.5%）为……。鹌鹑分离株的生长不依赖于烟酰胺腺嘌呤二核苷酸（NAD）（非NAD依赖型）。使用5株已鉴定的……分离株进行敏感性试验，结果显示它们对阿莫西林（AMC）和氨苄西林（AMP）100%敏感；对阿米卡星（AK）、红霉素（E）、庆大霉素（CN）和四环素（TE）100%耐药；对卡那霉素（K）和甲氧苄啶（W）80%耐药；对氯霉素（C）60%耐药；对恩诺沙星（ENR）20%耐药。具有中等敏感性（介于敏感和耐药之间）的抗生素是ENR和K，分别为80%和20%。使用HI试验，5株……分离株中有3株被鉴定为……的血清型B。
9株具有典型IC疾病症状的鹌鹑分离株中有5株（55.5%）被鉴定为……且对AMC和AMP敏感。5株……分离株中有3株被鉴定为血清型B。
