Fauziah Ima, Asmara Widya, Wahyuni Agnesia Endang Tri Hastuti
Student of Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Vet World. 2021 May;14(5):1124-1127. doi: 10.14202/vetworld.2021.1124-1127. Epub 2021 May 8.
Infectious coryza (IC) is an upper respiratory disease of chicken caused by . Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to determine the antimicrobial sensitivity of isolates from layers in the Special Region of Yogyakarta, Indonesia.
The samples used in this study were 30 layers that showed IC symptoms. The colony and cell morphology were observed with Gram staining; then, biochemical tests (catalase, oxidase, urease, indole, and motility tests, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) were performed to the suspected colony to identify . An antibiotic sensitivity test was performed using several antibiotic disks against isolates that were cultured on Mueller-Hinton Agar.
Out of 30 samples, 24 samples (80%) were found positive for . All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%). The antibiotics with intermediate sensitivity were enrofloxacin (79.2%), fosfomycin (75%), and ciprofloxacin (54.2%). The isolates were most resistant to erythromycin (100%), followed by tetracycline (87.5%), streptomycin (83.3%), doxycycline and kanamycin (70.8%), and trimethoprim (62.5%).
Out of the total samples, 24 samples (80%) from layers with IC symptoms were identified biochemically as . It was sensitive to AMP, AML, and C.
传染性鼻炎（IC）是由……引起的鸡的一种上呼吸道疾病。其临床症状为面部肿胀和有恶臭的鼻窦渗出物。本研究旨在确定来自印度尼西亚日惹特区蛋鸡的……分离株的抗菌敏感性。
本研究使用的样本是30只表现出IC症状的蛋鸡。通过革兰氏染色观察菌落和细胞形态；然后，对疑似菌落进行生化试验（过氧化氢酶、氧化酶、脲酶、吲哚和动力试验，以及使用乳糖、麦芽糖、甘露醇和山梨醇的碳水化合物发酵试验）以鉴定……。使用几种抗生素纸片对在穆勒-欣顿琼脂上培养的……分离株进行抗生素敏感性试验。
在30个样本中，24个样本（80%）被发现……呈阳性。所有分离株对氨苄西林（AMP）和阿莫西林（AML）（100%）以及氯霉素（C）（91.6%）敏感。中度敏感的抗生素是恩诺沙星（79.2%）、磷霉素（75%）和环丙沙星（54.2%）。分离株对红霉素耐药性最强（100%），其次是四环素（87.5%）、链霉素（83.3%）、强力霉素和卡那霉素（70.8%）以及甲氧苄啶（62.5%）。
在所有样本中，24个来自有IC症状蛋鸡的样本（80%）经生化鉴定为……。它对AMP、AML和C敏感。
