Antimicrobial sensitivity of isolates from layers in the special region of Yogyakarta, Indonesia.

BACKGROUND AND AIM

Infectious coryza (IC) is an upper respiratory disease of chicken caused by . Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to determine the antimicrobial sensitivity of isolates from layers in the Special Region of Yogyakarta, Indonesia.

MATERIALS AND METHODS

The samples used in this study were 30 layers that showed IC symptoms. The colony and cell morphology were observed with Gram staining; then, biochemical tests (catalase, oxidase, urease, indole, and motility tests, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) were performed to the suspected colony to identify . An antibiotic sensitivity test was performed using several antibiotic disks against isolates that were cultured on Mueller-Hinton Agar.

RESULTS

Out of 30 samples, 24 samples (80%) were found positive for . All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%). The antibiotics with intermediate sensitivity were enrofloxacin (79.2%), fosfomycin (75%), and ciprofloxacin (54.2%). The isolates were most resistant to erythromycin (100%), followed by tetracycline (87.5%), streptomycin (83.3%), doxycycline and kanamycin (70.8%), and trimethoprim (62.5%).

CONCLUSION

Out of the total samples, 24 samples (80%) from layers with IC symptoms were identified biochemically as . It was sensitive to AMP, AML, and C.