Hu Ruizhi, Tan Jijun, Li Zhanfeng, Wang Long, Shi Mingkun, Li Baizhen, Liu Ming, Yuan Xupeng, He Jianhua, Wu Xiaosong
Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Animal Science and Technology College, Beijing University of Agriculture, Beijing, China.
Front Nutr. 2022 Oct 27;9:1001031. doi: 10.3389/fnut.2022.1001031. eCollection 2022.
Placental function is vital to the fetal growth of sows, and resveratrol (RES) can protect cells against oxidative stress, which is one of the major factors impairing placental function. This study aimed to investigate the effect of dietary resveratrol (RES) on placental function and reproductive performance during late pregnancy in a sow model from the aspects of oxidative stress, insulin resistance, and gut microbiota. A total of 26 hybrid pregnant sows (Landrace × Yorkshire) with similar parity were randomly allocated into two groups ( = 13) and fed with a basal diet or a diet containing 200 mg/kg of resveratrol from day 85 of gestation until parturition. The dietary supplementation of RES increased the litter weight at parturition by 12.53% ( = 0.145), with ameliorated insulin resistance (HOMA-IR), increased triglyceride (TG) levels, and decreased interleukin (IL)-1β and IL-6 levels in serum ( < 0.05). Moreover, resveratrol increased the placental vascular density ( < 0.05) with the enhanced expression of nutrient transporter genes ( and ) and antioxidant genes, such as () and () but declined the expression of inflammatory genes, such as β and ( < 0.05). The characterization of the fecal microbiota revealed that resveratrol decreased the relative abundance of the and ( < 0.05), which had a positive linear correlation with the expression of β and ( < 0.05), but had a negative linear correlation with the expression of , and genes ( < 0.05). These data demonstrated that dietary supplementation with resveratrol can improve placental function with ameliorated insulin resistance, oxidative stress, and inflammation potentially by regulating and the in sows.
胎盘功能对母猪的胎儿生长至关重要，而白藜芦醇（RES）可以保护细胞免受氧化应激的影响，氧化应激是损害胎盘功能的主要因素之一。本研究旨在从氧化应激、胰岛素抵抗和肠道微生物群方面，探讨日粮白藜芦醇（RES）对母猪妊娠后期胎盘功能和繁殖性能的影响。将26头胎次相近的杂种妊娠母猪（长白×大白）随机分为两组（每组n = 13），从妊娠第85天至分娩分别饲喂基础日粮或含200 mg/kg白藜芦醇的日粮。日粮添加RES使分娩时仔猪窝重增加了12.53%（P = 0.145），改善了胰岛素抵抗（HOMA-IR），提高了血清甘油三酯（TG）水平，并降低了白细胞介素（IL）-1β和IL-6水平（P < 0.05）。此外，白藜芦醇增加了胎盘血管密度（P < 0.05），同时营养转运蛋白基因（SLC2A1和SLC7A11）以及抗氧化基因（如SOD1和CAT）的表达增强，但炎症基因（如TNF-β和IL-8）的表达下降（P < 0.05）。粪便微生物群特征分析表明，白藜芦醇降低了大肠杆菌和肠球菌的相对丰度（P < 0.05），它们与TNF-β和IL-8的表达呈正线性相关（P < 0.05），但与SLC2A1、SLC7A11、SOD1和CAT基因的表达呈负线性相关（P < 0.05）。这些数据表明，日粮添加白藜芦醇可以通过调节母猪肠道微生物群和肠道屏障功能来改善胎盘功能，减轻胰岛素抵抗、氧化应激和炎症反应。
