Gan Qingqin, Luan Mingbao, Hu Maolong, Liu Zhongsong, Zhang Zhenqian
College of Agriculture, Hunan Agricultural University, Changsha, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences/Key Laboratory of Stem-Fiber Biomass and Engineering Microbiology, Ministry of Agriculture, Changsha, China.
Front Plant Sci. 2022 Nov 3;13:1040511. doi: 10.3389/fpls.2022.1040511. eCollection 2022.
Sclerotinia disease and weeds of greatly reduce crop yields. However, brassinolides can improve the resistance of plants to sclerotinia diseases and herbicides. In this study, we investigated the effects of brassinolide on the occurrence, physiological indices, yield, and gene expression of Fanming No. 1 seeds under sclerotinia and glufosinate stress. The results showed that soaking of the seeds in 0.015% brassinolide for 6 h reduced the incidence of sclerotinia by 10%. Additionally, in response to glufosinate stress at the seedling stage, the enzyme activities of catalase and superoxide dismutase increased by 9.6 and 19.0 U/gFW/min, respectively, and the soluble sugar content increased by 9.4 mg/g, increasing the stress resistance of plants and yield by 2.4%. , , , , , , , and were screened by transcriptome analysis. and were identified as key genes. Following glufosinate treatment, transgenic plants overexpressing ALDH3F1 and CYP90A1 were found to be resistant to glufosinate, and the expression levels of the and were 1.03-2.37-fold as high as those in the control. The expression level of , which is an antibacterial gene related to sclerotinia disease, in transgenic plants was 2.40-2.37-fold as high as that in the control. Our results indicate that these two key genes promote plant resistance to sclerotinia and glufosinate. Our study provides a foundation for further studies on the molecular mechanisms of rapeseed resistance breeding and selection of new resistant varieties.
菌核病和杂草会大幅降低作物产量。然而，油菜素内酯可以提高植物对菌核病和除草剂的抗性。在本研究中，我们调查了油菜素内酯对菌核病和草铵膦胁迫下泛明1号种子的发病情况、生理指标、产量及基因表达的影响。结果表明，将种子在0.015%油菜素内酯中浸泡6小时可使菌核病发病率降低10%。此外，在幼苗期应对草铵膦胁迫时，过氧化氢酶和超氧化物歧化酶的酶活性分别提高了9.6和19.0 U/gFW/min，可溶性糖含量增加了9.4 mg/g，使植物的抗逆性和产量提高了2.4%。通过转录组分析筛选出了……、……、……、……、……、……、……和……。并将……和……鉴定为关键基因。在草铵膦处理后，发现过表达ALDH3F1和CYP90A1的转基因植物对草铵膦具有抗性，且……和……的表达水平是对照的1.03 - 2.37倍。作为与菌核病相关的抗菌基因，……在转基因植物中的表达水平是对照的2.40 - 2.37倍。我们的结果表明，这两个关键基因促进了植物对菌核病和草铵膦的抗性。我们的研究为进一步研究油菜抗性育种的分子机制和选育新的抗性品种奠定了基础。
