Zhang Binbin, Zhu Shuaipeng, He Yuehua, Liang Wenjie, Zhu Tingqi, Han Ruili, Li Donghua, Wang Yanbin, Tian Yadong, Li Guoxi, Kang Xiangtao, Li Wenting, Sun Guirong
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
The Shennong Seed Industry Laboratory, Zhengzhou 450002, China.
Animals (Basel). 2025 Jun 25;15(13):1879. doi: 10.3390/ani15131879.
Intramuscular fat content is positively correlated with meat flavor and juiciness. Increasing the intramuscular fat (IMF) content of chickens while increasing their growth rate has become a hot topic in molecular breeding. The group's previous studies showed that miR-128-3p inhibited chicken intramuscular adipocyte differentiation and lipogenesis. However, the regulatory mechanism of miR-128-3p in intramuscular preadipocytes is currently unknown. In this study, we investigated the mechanism of miR-128-3p regulation of chicken intramuscular adipocyte differentiation and deposition. Transcriptome data analysis of differential LincRNAs indicated that, compared to the NC group, the mimics-treated group had seventeen significantly differentially expressed LincRNAs ( < 0.05), including six upregulated and eleven downregulated ones; the inhibitor-treated group had seventeen differentially expressed LincRNAs ( < 0.05), including eight upregulated and nine downregulated ones; and twenty-four differentially expressed LincRNAs ( < 0.05) were observed when comparing the mimics-treated group to the inhibitor-treated group, with fourteen upregulated and ten downregulated ones. Functional enrichment analysis revealed that DELincRNAs from the overexpression group (M group) and interference group (SI group) were involved in the negative regulation of metabolic processes, response to steroid hormones, and regulation of actin cytoskeleton. Furthermore, target gene prediction analysis showed that miR-128-3p can target many of the DELincRNAs, such as LincRNA-MSTRG.673.2, LincRNA-MSTRG.39.2, LincRNA-MSTRG.39.3, and LincRNA-MSTRG.14270.2. LincRNA-MSTRG.673.2 was predominantly expressed in the cytoplasm of intramuscular adipocytes. Dual luciferase reporter identified the targeting relationship between miR-128-3p and LincRNA-MSTRG.673.2. The results of subsequent functional assays demonstrated that interfering with MSTRG.673.2 has been shown to inhibit lipid deposition in intramuscular preadipocytes. Transfection experiments have shown that LincRNA-MSTRG.673.2 can affect the expression of miR-128-3p. This study found that LincRNA-MSTRG.673.2 promoted chicken intramuscular adipocyte differentiation by downregulating miR-128-3p. The results are noteworthy for improving chicken meat quality, molecular breeding, and lipid metabolism research.
肌内脂肪含量与肉的风味和多汁性呈正相关。在提高鸡生长速度的同时增加其肌内脂肪（IMF）含量已成为分子育种领域的一个热门话题。该团队之前的研究表明，miR-128-3p抑制鸡肌内脂肪细胞的分化和脂肪生成。然而，miR-128-3p在肌内前体细胞中的调控机制目前尚不清楚。在本研究中，我们探究了miR-128-3p调控鸡肌内脂肪细胞分化和沉积的机制。差异LincRNAs的转录组数据分析表明，与NC组相比，模拟物处理组有17个显著差异表达的LincRNAs（<0.05），包括6个上调和11个下调的；抑制剂处理组有17个差异表达的LincRNAs（<0.05），包括8个上调和9个下调的；当比较模拟物处理组和抑制剂处理组时，观察到24个差异表达的LincRNAs（<0.05），其中14个上调，10个下调。功能富集分析显示，过表达组（M组）和干扰组（SI组）的差异表达LincRNAs（DELincRNAs）参与了代谢过程的负调控、对类固醇激素的反应以及肌动蛋白细胞骨架的调控。此外，靶基因预测分析表明，miR-128-3p可以靶向许多DELincRNAs，如LincRNA-MSTRG.67（查看全文）
