Yu Jinyan, Sun Min, Wang Xiaoyu, Qi Dongmei, Han Chunchao
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, China.
Experimental center, Shandong University of Traditional Chinese Medicine, Jinan, China.
Biomed Chromatogr. 2023 Feb;37(2):e5551. doi: 10.1002/bmc.5551. Epub 2022 Dec 4.
Cordycepin is an important quality control marker in Cordyceps militaris. This study aimed to explain the metabolic mechanisms for high-yielding cordycepin of C. militaris. In this study, high-yielding strains of cordycepin were obtained by ultraviolet mutagenesis, and the polysaccharide and protein contents were also changed. In high-yielding strains, the protein content significantly increased, whereas the polysaccharide content decreased. Simultaneously, metabolic differences for high- and low-yielding cordycepin strains were detected by metabolomics. Metabolomics results showed that the relative content of most metabolites decreased in high-yielding cordycepin strains. Various metabolic pathways have been altered in high-yielding cordycepin strains, such as the citric acid cycle, purine metabolism, and pyrimidine metabolism, leading to an increase in cordycepin content. In addition, changes in metabolic poly-pathways related to polysaccharide and protein synthesis, such as galactose metabolism and amino acid metabolism, promoted an increase in cordycepin content. This study analyzes the high yield of cordycepin in C. militaris at the metabolic level and provides a theoretical basis for further increasing cordycepin content.
虫草素是蛹虫草中的一个重要质量控制指标。本研究旨在阐释蛹虫草虫草素高产的代谢机制。在本研究中，通过紫外线诱变获得了虫草素高产菌株，且多糖和蛋白质含量也发生了变化。在高产菌株中，蛋白质含量显著增加，而多糖含量降低。同时，采用代谢组学方法检测了虫草素高产和低产菌株的代谢差异。代谢组学结果显示，在虫草素高产菌株中，大多数代谢物的相对含量下降。虫草素高产菌株中的各种代谢途径发生了改变，如柠檬酸循环、嘌呤代谢和嘧啶代谢，从而导致虫草素含量增加。此外，与多糖和蛋白质合成相关的代谢多途径变化，如半乳糖代谢和氨基酸代谢，也促进了虫草素含量的增加。本研究从代谢水平分析了蛹虫草中虫草素的高产情况，为进一步提高虫草素含量提供了理论依据。
