International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry & Technology, Changsha, Hunan 410004, China; Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry & Technology, Changsha, Hunan 410004, China; Microbial Variety Creation Center, Hunan Provincial Laboratory of Yuelushan Seed Industry, Changsha 410004, China.
International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry & Technology, Changsha, Hunan 410004, China; Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry & Technology, Changsha, Hunan 410004, China.
Bioresour Technol. 2023 Apr;373:128705. doi: 10.1016/j.biortech.2023.128705. Epub 2023 Feb 4.
Cordycepin is the key pharmacologically active compound of Cordyceps militaris, and various fermentation strategies have been developed to increase cordycepin production. This study aimed to investigate the effect of rotenone on cordycepin biosynthesis in submerged fermentation of C. militaris, and also to explore its possible induction mechanisms via multi-omics analysis. Adding 5 mg/L rotenone significantly increased the cordycepin production by 316.09 %, along with mycelial growth inhibition and cell wall destruction. Moreover, transcriptomic analysis and metabolomic analysis revealed the accumulation of cordycepin was promoted by alterations in energy metabolism and amino acid metabolism pathways. Finally, the integration analysis of the two omics confirmed rotenone altered the nucleotide metabolism pathway toward adenosine and up-regulated the cordycepin synthesis genes (cns1-3) to convert adenosine to cordycepin. This work reports, for the first time, rotenone could act as an effective inducer of cordycepin synthesis.
蛹虫草中的关键药效活性化合物是虫草素，目前已经开发出了多种发酵策略来提高虫草素的产量。本研究旨在探讨鱼藤酮对蛹虫草液体发酵过程中虫草素生物合成的影响，并通过多组学分析来探索其可能的诱导机制。添加 5mg/L 的鱼藤酮可使虫草素的产量显著提高 316.09%，同时抑制菌丝生长并破坏细胞壁。此外，转录组学分析和代谢组学分析表明，虫草素的积累是通过改变能量代谢和氨基酸代谢途径来促进的。最后，对这两种组学的综合分析证实，鱼藤酮改变了核苷酸代谢途径，使腺苷向虫草素转化，并上调了虫草素合成基因（cns1-3），将腺苷转化为虫草素。本研究首次报道，鱼藤酮可以作为一种有效的虫草素合成诱导剂。
