An electrochemical sensor based on AuPd@FeO nanozymes for a sensitive and quantitative detection of hydrogen peroxide in real samples.

The hydrogen peroxide (HO) levels in living organisms and environment have strong effects on many biological processes inducing cell apoptosis/cell necrosis and wound disinfection. Therefore, it is important to have an accurate and detection of HO. Herein, an AuPd@FeO nanozyme-based electrochemical (EC) sensor (termed as AuPd@FeO NPs/GCE) with good stability and anti-interference ability has been prepared for the detection of HO by differential pulse voltammetry (DPV) and chronoamperometry dual-measurement modes. The AuPd@FeO NPs/GCE exhibits good linear relationships in the ranges from 13.0 to 6.0 × 10 μM (DPV measurement) and 50 to 1.0 × 10 μM (chronoamperometry measurement), low detection limits (LODs) of 1.6 μM (DPV measurement) and 3.0 μM (chronoamperometry measurement) and high sensitivities of 83.8 nA μM cm (DPV measurement) and 120.7 nA μM cm (chronoamperometry measurement). The practicability of the as-prepared AuPd@FeO NPs/GCE has been demonstrated by an real-time detection of HO released from adherent living MCF-7 cells triggered by varying amounts of -formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) from 0.5 to 3.0 μM and the quantitative determination of HO in commercial disinfectants.