State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
University of Science and Technology of China, Hefei 230026, China.
Analyst. 2022 Dec 5;147(24):5739-5746. doi: 10.1039/d2an01509a.
The hydrogen peroxide (HO) levels in living organisms and environment have strong effects on many biological processes inducing cell apoptosis/cell necrosis and wound disinfection. Therefore, it is important to have an accurate and detection of HO. Herein, an AuPd@FeO nanozyme-based electrochemical (EC) sensor (termed as AuPd@FeO NPs/GCE) with good stability and anti-interference ability has been prepared for the detection of HO by differential pulse voltammetry (DPV) and chronoamperometry dual-measurement modes. The AuPd@FeO NPs/GCE exhibits good linear relationships in the ranges from 13.0 to 6.0 × 10 μM (DPV measurement) and 50 to 1.0 × 10 μM (chronoamperometry measurement), low detection limits (LODs) of 1.6 μM (DPV measurement) and 3.0 μM (chronoamperometry measurement) and high sensitivities of 83.8 nA μM cm (DPV measurement) and 120.7 nA μM cm (chronoamperometry measurement). The practicability of the as-prepared AuPd@FeO NPs/GCE has been demonstrated by an real-time detection of HO released from adherent living MCF-7 cells triggered by varying amounts of -formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) from 0.5 to 3.0 μM and the quantitative determination of HO in commercial disinfectants.
生物体和环境中的过氧化氢 (HO) 水平对许多诱导细胞凋亡/坏死和伤口消毒的生物过程有很强的影响。因此，准确检测 HO 非常重要。在此，我们制备了一种基于 AuPd@FeO 纳米酶的电化学 (EC) 传感器 (称为 AuPd@FeO NPs/GCE)，该传感器具有良好的稳定性和抗干扰能力，可通过差分脉冲伏安法 (DPV) 和计时安培法双测量模式检测 HO。AuPd@FeO NPs/GCE 在 13.0 至 6.0×10 μM（DPV 测量）和 50 至 1.0×10 μM（计时安培法测量）的范围内呈现出良好的线性关系，检测限 (LOD) 低至 1.6 μM（DPV 测量）和 3.0 μM（计时安培法测量），灵敏度高，分别为 83.8 nA μM cm（DPV 测量）和 120.7 nA μM cm（计时安培法测量）。通过实时检测不同浓度 -formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) 从 0.5 到 3.0 μM 诱导贴壁 MCF-7 细胞释放的 HO，以及定量测定商业消毒剂中的 HO，证明了所制备的 AuPd@FeO NPs/GCE 的实用性。
