College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, PR China.
Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital), Qingdao, 266000, PR China.
Talanta. 2024 Jan 1;266(Pt 2):125132. doi: 10.1016/j.talanta.2023.125132. Epub 2023 Aug 28.
A novel flexible electrochemical sensor based on porous carbon nanosheets (PCNSs) nanozyme has been constructed for in situ and real-time monitoring of HO released by cells. The PCNSs are prepared with the integration of thermal transformation, thermal activation and sonochemical exfoliation by using zeolitic imidazolate frameworks as template. The PCNSs exhibit high electrical conductivity, electrochemical activity and peroxidase-like catalytic properties, which is beneficial to HO assay. With the transfer printing method, the flexible electrochemical sensor is obtained, which has excellent performances for HO electrochemical detecting with wide linear range from 1 μM to 20 mM and a low detection limit of 0.76 μM. Owing to the great biocompatibility, the flexible sensor guarantees the growth of living cells for 72 h and realizes in situ and real-time monitoring the release of HO from HeLa cells. The strategy of porous nanozyme preparation and flexible sensor construction provided a promising way for in situ and real-time assay of small molecules in the cellular microenvironment.
一种基于多孔碳纳米片（PCNSs）纳米酶的新型柔性电化学传感器已被构建，用于原位和实时监测细胞释放的 HO。PCNSs 通过沸石咪唑酯骨架作为模板的热转化、热激活和超声化学剥离来制备。PCNSs 表现出高导电性、电化学活性和过氧化物酶样催化性能，有利于 HO 分析。通过转印印刷法，获得了具有优异性能的柔性电化学传感器，其对 HO 的电化学检测具有较宽的线性范围（1 μM 至 20 mM）和较低的检测限（0.76 μM）。由于具有很好的生物相容性，柔性传感器保证了活细胞生长 72 h，并实现了原位和实时监测 HeLa 细胞中 HO 的释放。这种多孔纳米酶制备和柔性传感器构建的策略为细胞微环境中小分子的原位和实时分析提供了一种很有前途的方法。
