Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304.
Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Proc Natl Acad Sci U S A. 2022 Nov 29;119(48):e2206829119. doi: 10.1073/pnas.2206829119. Epub 2022 Nov 21.
Retinal ganglion cells (RGCs) are heterogeneous projection neurons that convey distinct visual features from the retina to brain. Here, we present a high-throughput in vivo RGC activity assay in response to light stimulation using noninvasive Ca imaging of thousands of RGCs simultaneously in living mice. Population and single-cell analyses of longitudinal RGC Ca imaging reveal distinct functional responses of RGCs and unprecedented individual RGC activity conversions during traumatic and glaucomatous degeneration. This study establishes a foundation for future in vivo RGC function classifications and longitudinal activity evaluations using more advanced imaging techniques and visual stimuli under normal, disease, and neural repair conditions. These analyses can be performed at both the population and single-cell levels using temporal and spatial information, which will be invaluable for understanding RGC pathophysiology and identifying functional biomarkers for diverse optic neuropathies.
视网膜神经节细胞（RGCs）是一种异质性的投射神经元，它们将不同的视觉特征从视网膜传递到大脑。在这里，我们使用活体小鼠中数千个 RGC 的非侵入性 Ca 成像，展示了一种针对光刺激的高通量体内 RGC 活性测定方法。对纵向 RGC Ca 成像的群体和单细胞分析揭示了 RGC 的不同功能反应，以及在创伤性和青光眼变性过程中前所未有的个体 RGC 活性转换。这项研究为未来使用更先进的成像技术和视觉刺激在正常、疾病和神经修复条件下进行体内 RGC 功能分类和纵向活性评估奠定了基础。这些分析可以在群体和单细胞水平上使用时间和空间信息来进行，这对于理解 RGC 的病理生理学和确定各种视神经病变的功能生物标志物将是非常有价值的。
