The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, 2800, Denmark.
Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kgs. Lyngby, 2800, Denmark.
Adv Healthc Mater. 2023 Feb;12(4):e2201897. doi: 10.1002/adhm.202201897. Epub 2022 Dec 4.
During the past decades, microdevices have been evaluated as a means to overcome challenges within oral drug delivery, thus improving bioavailability. Fabrication of microdevices is often limited to planar or simple 3D designs. Therefore, this work explores how microscale stereolithography 3D printing can be used to fabricate radiopaque microcontainers with enhanced mucoadhesive geometries, which can enhance bioavailability by increasing gastrointestinal retention. Ex vivo force measurements suggest increased mucoadhesion of microcontainers with adhering features, such as pillars and arrows, compared to a neutral design. In vivo studies, utilizing planar X-ray imaging, show the time-dependent gastrointestinal location of microcontainers, whereas computed tomography scanning and cryogenic scanning electron microscopy reveal information about their spatial dynamics and mucosal interactions, respectively. For the first time, the effect of 3D microdevice modifications on gastrointestinal retention is traced in vivo, and the applied methods provide a much-needed approach for investigating the impact of device design on gastrointestinal retention.
在过去的几十年中，微器件已被评估为克服口服药物输送中挑战的一种手段，从而提高生物利用度。微器件的制造通常限于平面或简单的 3D 设计。因此，这项工作探索了微尺度立体光刻 3D 打印如何可用于制造具有增强的粘液附着几何形状的射线可检测微容器，通过增加胃肠道保留时间来提高生物利用度。体外力测量表明，与中性设计相比，具有附着特征（如柱子和箭头）的微容器具有更高的粘液附着性。体内研究利用平面 X 射线成像显示了微容器在胃肠道中的时间依赖性位置，而计算机断层扫描和低温扫描电子显微镜则分别提供了有关其空间动力学和粘膜相互作用的信息。首次在体内追踪了 3D 微器件修改对胃肠道保留的影响，所应用的方法为研究器件设计对胃肠道保留的影响提供了急需的方法。
