Gao Wei-Na, Bian Xiang-Yu, Xu Qin-Gao, Dong Wei-Yun, Ma Yu-Ying, Guo Chang-Jiang
Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Jul;38(4):308-312. doi: 10.12047/j.cjap.6307.2022.058.
To investigate the anti-fatigue effects of composition of Moringa oleifera leaves and Polygonatum polysaccharide, and to explore the mechanisms. Thirty male Kunming mice were randomly divided into control (C) and composition of Moringa oleifera leaves and Polygonatum polysaccharide group (MP). There were 15 mice in each group. Group C was given distilled water and the group MP was given composition intragastriclly every day. The volume was 0.5 ml. After 14 days of treatment, weight-bearing swimming experiment was conducted, and exhaustive swimming time was recorded. The bearing weight was 3% of the body weight. In another experiment, 48 male Kunming mice were randomly divided into quiet control group (QC), swimming control group (SC) and composition group (MP). There were 16 mice in each group. The QC and SC groups were given distilled water intragastrically, and the group MP was treated with composition every day for 14 days. The volume was 0.5 ml. On the day 15, 30 minutes after intragastriclly administration of distilled water, blood, liver and hind leg muscle of the QC group were collected immediately. The SC and MP groups were subjected non-weight-bearing swimming experiment, and blood, liver and hind leg muscle were collected after swimming. The fatigue related indexes, oxidant/antioxidant parameters and energy metabolism indicators in serum and tissues were determined by commercial kits. The exhaustive swimming time of mice in MP group was significantly longer than that in the C group (P<0.05). Compared with the control group, non-weight-bearing swimming decreased the contents of serum glucose and GSH, the contents of hepatic glycogen and ATP, the hepatic activities of SOD, LDH and ATPase, and muscle activity of GSH-Px (P< 0.05). However, serum levels of BUN and MDA were increased (P<0.05). Compared with the SC group, the composition remarkably increased the contents of serum glucose and hepatic glycogen, increased serum content of GSH, enhanced hepatic activities of SOD, LDH and ATPase and muscle activity of GSH-Px, and increased the hepatic content of ATP (P<0.05). However, the serum level of BUN was decreased (P<0.05). The Moringa oleifera leaves and Polygonatum polysaccharide composition possesses anti-fatigue effects. Anti-oxidant and improving energy metabolism could be the important mechanisms.
探讨辣木叶与黄精多糖组合物的抗疲劳作用及其机制。将30只雄性昆明小鼠随机分为对照组（C）和辣木叶与黄精多糖组合物组（MP），每组15只。C组给予蒸馏水，MP组每天灌胃给予组合物，体积为0.5 ml。处理14天后，进行负重游泳实验，记录小鼠的力竭游泳时间，负重为体重的3%。在另一个实验中，将48只雄性昆明小鼠随机分为安静对照组（QC）、游泳对照组（SC）和组合物组（MP），每组16只。QC组和SC组灌胃给予蒸馏水，MP组每天给予组合物处理14天，体积为0.5 ml。在第15天，QC组灌胃蒸馏水30分钟后，立即采集血液、肝脏和后肢肌肉。SC组和MP组进行无负重游泳实验，游泳结束后采集血液、肝脏和后肢肌肉。采用商业试剂盒测定血清和组织中与疲劳相关的指标、氧化/抗氧化参数以及能量代谢指标。MP组小鼠的力竭游泳时间显著长于C组（P<0.05）。与对照组相比，无负重游泳降低了血清葡萄糖和谷胱甘肽（GSH）的含量、肝糖原和三磷酸腺苷（ATP）的含量、肝脏中超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）和ATP酶的活性以及肌肉中谷胱甘肽过氧化物酶（GSH-Px）的活性（P<0.05）。然而，血清尿素氮（BUN）和丙二醛（MDA）水平升高（P<0.05）。与SC组相比，该组合物显著增加了血清葡萄糖和肝糖原的含量，提高了血清GSH含量，增强了肝脏中SOD、LDH和ATP酶的活性以及肌肉中GSH-Px的活性，并增加了肝脏中ATP的含量（P<0.05）。然而，血清BUN水平降低（P<0.05）。辣木叶与黄精多糖组合物具有抗疲劳作用。抗氧化和改善能量代谢可能是其重要机制。
