由GATA蛋白反式激活的OCT4表达对于非啮齿动物滋养外胚层早期发育至关重要。

OCT4 expression transactivated by GATA protein is essential for non-rodent trophectoderm early development.

作者信息

Bou Gerelchimeg, Guo Jia, Liu Shichao, Guo Shimeng, Davaakhuu Gantulga, Lv Qi, Xue Binghua, Qiao Shupei, Lv Jiawei, Weng Xiaogang, Zhao Jianchao, Zhang Yuting, He Yilong, Zhang Heng, Chai Zhuang, Liu Yan, Yu Yanbo, Qu Bo, Sun Ruizhen, Shen Xinghui, Lei Lei, Liu Zhonghua

机构信息

College of Life Science, Northeast Agricultural University, Harbin 150030, China; College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.

College of Life Science, Northeast Agricultural University, Harbin 150030, China.

出版信息

Cell Rep. 2022 Nov 22;41(8):111644. doi: 10.1016/j.celrep.2022.111644.

DOI:10.1016/j.celrep.2022.111644

PMID:36417852

Abstract

Oct4 is exclusively expressed in rodent inner cell mass (ICM) but silenced in its trophectoderm (TE). However, for many non-rodent animals, including pig, cattle, rabbit, goat, and human, OCT4 has a remarkable expression in early TE. This study, applying pig as the main research model, proves that OCT4 expression in TE is supported by a unique GATA motif in the OCT4 upstream conserved regulatory region, and GATA4 is responsible for its activation. Moreover, OCT4 acts as a specific regulator of a narrow range of genes (including BCL2A1 and HNRNP2AB1) that are essential for the first wave of rapid proliferation in early TE. This study describes the regulatory mechanism to direct the OCT4 expression and its significance in TE of porcine preimplantation embryo.

摘要

Oct4仅在啮齿动物的内细胞团（ICM）中表达，而在其滋养外胚层（TE）中沉默。然而，对于许多非啮齿动物，包括猪、牛、兔、山羊和人类，OCT4在早期滋养外胚层中具有显著表达。本研究以猪为主要研究模型，证明了滋养外胚层中OCT4的表达由OCT4上游保守调控区域中一个独特的GATA基序所支持，且GATA4负责其激活。此外，OCT4作为一系列特定基因（包括BCL2A1和HNRNP2AB1）的特异性调节因子，这些基因对于早期滋养外胚层的第一波快速增殖至关重要。本研究描述了指导猪植入前胚胎滋养外胚层中OCT4表达的调控机制及其意义。