Zhang Huan, Cheng Qin, Wang Xu, Jia Wei, Xie Jiatao, Fan Guocheng, Han Chuang, Zhao Xiaohu
State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
Institute of Quality Standard and Monitoring Technology for Agro-Products of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
J Fungi (Basel). 2022 Nov 11;8(11):1193. doi: 10.3390/jof8111193.
is a broad-spectrum necrotrophic phytopathogen that can infect many plant species worldwide. The application of fungicides is a common measure for controlling . Due to the risk of developing resistance to fungicides, it is imperative to find ways to be environmentally friendly and even effective. Using bioactive compounds in plants to reduce the amounts of fungicides has become a clean and sustainable strategy of controlling . Our study found that selenium in soil mediated the phenylacetic acid-related metabolic pathway in oilseed rape and reduced the incidence rate of . The growth-inhibition rates of were observed at 25.82%, 19.67%, and 52.61% for treatments of 0.8 mg·L dimethachlon, 0.1 mg·mL phenylacetic acid, and dimethachlon (0.8 mg·L) + phenylacetic acid (0.1 mg·mL), respectively. Phenylacetic acid reduced the application amount of dimethachlon and enhanced the inhibition effect for . Results also suggested that phenylacetic acid severely damaged the morphological structure, changed the electrical conductivity, and reduced the capacity of acid production and oxalic acid secretion of mycelium. Further studies revealed that phenylacetic acid increased the gene-expression level of , , and in mycelium while decreasing the expression level of , and phenylacetic acid + dimethachlon reduced the relative expression level of . These findings verified that phenylacetic acid could partially replace the amount of dimethachlon, as well as enhance the prevention of by dimethachlon, which provides evidence for developing an environment-friendly method for control.
是一种广谱坏死性植物病原菌，可感染全球许多植物物种。施用杀菌剂是控制该病原菌的常用措施。由于存在对杀菌剂产生抗性的风险，因此必须找到既环保又有效的方法。利用植物中的生物活性化合物来减少杀菌剂用量已成为一种清洁且可持续的控制该病原菌的策略。我们的研究发现，土壤中的硒介导了油菜中与苯乙酸相关的代谢途径，并降低了该病原菌的发病率。对于0.8 mg·L哒螨灵、0.1 mg·mL苯乙酸以及哒螨灵（0.8 mg·L）+苯乙酸（0.1 mg·mL）处理，该病原菌的生长抑制率分别为25.82%、19.67%和52.61%。苯乙酸减少了哒螨灵的施用量，并增强了对该病原菌的抑制效果。结果还表明，苯乙酸严重破坏了该病原菌菌丝体的形态结构，改变了电导率，并降低了产酸和草酸分泌能力。进一步研究表明，苯乙酸提高了该病原菌菌丝体中、、和的基因表达水平，同时降低了的表达水平，而苯乙酸+哒螨灵降低了的相对表达水平。这些发现证实，苯乙酸可以部分替代哒螨灵的用量，同时增强哒螨灵对该病原菌的防治效果，这为开发一种环境友好型的该病原菌控制方法提供了证据。
