Chang Che-Kang, Yang Min-Chi, Chen Hsueh-Fen, Liao Yi-Ling, Lan Chung-Yu
Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan.
Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan.
J Fungi (Basel). 2022 Nov 13;8(11):1196. doi: 10.3390/jof8111196.
The cell wall is the first interface for interaction with the surrounding environment and the host cells. Therefore, maintenance of cell wall integrity (CWI) is crucial for survival and host-pathogen interaction. In response to environmental stresses, undergoes cell wall remodeling controlled by multiple signaling pathways and transcription regulators. Here, we explored the role of the transcription factor Sfp1 in CWI. A deletion of the gene not only caused changes in cell wall properties, cell wall composition and structure but also modulated expression of cell wall biosynthesis and remodeling genes. In addition, Cas5 is a known transcription regulator for CWI and cell wall stress response. Interestingly, our results indicated that Sfp1 negatively controls the gene expression by binding to its promoter element. Together, this study provides new insights into the regulation of CWI and stress response.
细胞壁是与周围环境和宿主细胞相互作用的首个界面。因此，维持细胞壁完整性（CWI）对于生存和宿主-病原体相互作用至关重要。响应环境应激，细胞壁会经历由多种信号通路和转录调节因子控制的重塑过程。在此，我们探究了转录因子Sfp1在CWI中的作用。该基因的缺失不仅导致细胞壁特性、细胞壁组成和结构发生变化，还调节了细胞壁生物合成和重塑基因的表达。此外，Cas5是已知的CWI和细胞壁应激反应的转录调节因子。有趣的是，我们的结果表明，Sfp1通过结合其启动子元件负向控制该基因的表达。总之，本研究为CWI和应激反应的调控提供了新的见解。
