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一种新型多糖对 Pb、Cu 和 Cd 的生物吸附特性及细胞保护作用。

Biosorption Characteristic and Cytoprotective Effect of Pb, Cu and Cd by a Novel Polysaccharide from .

机构信息

Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.

College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.

出版信息

Molecules. 2022 Nov 19;27(22):8036. doi: 10.3390/molecules27228036.

Abstract

The pollution of heavy metal ions can cause damage to the human body through food, so developing a new biocompatible material that can remove the damage of heavy metal ions has a good application prospect. In this study, we obtained a new homogeneous polysaccharide composed of seven monosaccharides from by using the method of separation and purification of polysaccharide. The results of adsorption behavior showed that the concentration, temperature and pH value could affect the adsorption effect of polysaccharide (ZSP). Through model fitting of the data of adsorption time and metal concentration, the pseudo second-order kinetic model can well describe the kinetics of the adsorption process, and the adsorption isotherm data fit well with the Langmuir model. In the preliminary research results of adsorption mechanism, SEM showed the appearance of ZSP as flake and porous surface; EDX analysis confirmed the metal adsorption capacity of ZSP. Meanwhile, XPS and FT-IR further clarified the adsorption based on functional groups composed of C and O. The cells preprotected by ZSP can resist heavy metal ions. The above results show that ZSP can be used as a new macromolecule to bind heavy metal ions, which can broaden the research scope of polysaccharides in contaminated food systems.

摘要

重金属离子的污染会通过食物对人体造成损害,因此开发一种新的生物相容性材料来去除重金属离子的损害具有很好的应用前景。在这项研究中,我们使用多糖的分离和纯化方法,从 中获得了一种由七种单糖组成的新型均相多糖。吸附行为的结果表明,浓度、温度和 pH 值会影响 多糖 (ZSP) 的吸附效果。通过对吸附时间和金属浓度数据的模型拟合,准二级动力学模型可以很好地描述吸附过程的动力学,吸附等温线数据很好地符合朗缪尔模型。在吸附机制的初步研究结果中,SEM 显示 ZSP 的外观为片状和多孔表面;EDX 分析证实了 ZSP 的金属吸附能力。同时,XPS 和 FT-IR 进一步阐明了基于 C 和 O 组成的功能基团的吸附。经 ZSP 预先保护的细胞可以抵抗重金属离子。上述结果表明,ZSP 可以用作结合重金属离子的新型大分子,这可以拓宽多糖在污染食品系统中的研究范围。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de3f/9696034/b717bbbff097/molecules-27-08036-g001.jpg

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