State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, PR China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Int J Biol Macromol. 2020 Dec 15;165(Pt B):1900-1910. doi: 10.1016/j.ijbiomac.2020.10.127. Epub 2020 Oct 21.
A novel polysaccharide from Siraitia grosvenorii residues (SGP, molecular weight 1.93 × 10 KDa) was isolated and purified. SGP was composed of α-L-Arabinose, α-D-Mannose, α-d-Glucose, α-D-Galactose, Glucuronic acid, and Galacturonic acid with the ratio of 1: 1.92: 3.98: 7.63: 1.85: 7.34. The backbone of SGP was consist of galactoses and linked by α-(1,4)-glycosidic bond. The branch chains including α-1,6 linked glucose branch, α-1,6 linked mannose branch, α-1,3 linked galactose branch and arabinose branched (α-L-Ara(1→). The results of bioactivity experiments suggested that SGP had antioxidant in vitro, especially on scavenging DPPH radicals. Besides, SGP resulted in the decrease of ROS and the percentage of apoptotic and necrotic cells in a dose-dependent manner in HO oxide injury PC12 cells. This research could help to develop the potential value and utilization of Siraitia grosvenorii.
从罗汉果废渣中分离得到一种新型多糖（SGP，分子量为 1.93×10 KDa）。SGP 由 α-L-阿拉伯糖、α-D-甘露糖、α-d-葡萄糖、α-D-半乳糖、葡萄糖醛酸和半乳糖醛酸组成，摩尔比为 1:1.92:3.98:7.63:1.85:7.34。SGP 的主链由半乳糖组成，通过 α-(1,4)-糖苷键连接。支链包括 α-1,6 连接的葡萄糖支链、α-1,6 连接的甘露糖支链、α-1,3 连接的半乳糖支链和阿拉伯糖支链（α-L-Ara(1→)。活性实验结果表明，SGP 具有体外抗氧化活性，特别是对 DPPH 自由基的清除能力。此外，SGP 可降低 HO 诱导的 PC12 细胞中 ROS 的产生，并呈剂量依赖性降低细胞凋亡和坏死的比例。本研究有助于进一步开发罗汉果的潜在价值和利用。
