Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran.
Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 25457, South Korea.
Carbohydr Polym. 2020 Feb 15;230:115636. doi: 10.1016/j.carbpol.2019.115636. Epub 2019 Nov 18.
The aim of the present study was to evaluate the effect of water-soluble polysaccharides from Cuminum cyminum to induce inflammatory response in immune cells and understand their underlying mechanisms. Weight average molecular weight (M) of polysaccharides varied between 191.4-512.2 × 10 g/mol. Polysaccharides induced RAW264.7 cells to release nitric oxide and express TNF-α, IL-1β, IL-6 and IL-12 inflammatory cytokines. Polysaccharides activated NK-92 cells to produce TNF-α, IFN-γ, perforin, granzyme B, NKG2D and FasL. Activations of RAW264.7 and NK-92 cells were through NF-κB and MAPKs signal pathways indicated by the presence of phosphorylated NF-κB, ERK, JNK and p38 proteins. The polysaccharide structure was mainly constituted of →4)-Galp-(1→, →3)-Galp-(1→, →2)-Arap-(1→ and →2)-Arap-(1→ glycosidic linkages. Overall results suggested that polysaccharides from C. cyminum possessing lower M and greater expanded conformation more effectively stimulate RAW264.7 and NK-92 cells and thus could be considered for further studies on their biomedical applications.
本研究旨在评估孜然水溶性多糖诱导免疫细胞炎症反应的效果，并探讨其潜在机制。多糖的重均分子量（Mw）在 191.4-512.2×10g/mol 之间变化。多糖诱导 RAW264.7 细胞释放一氧化氮，并表达 TNF-α、IL-1β、IL-6 和 IL-12 等炎症细胞因子。多糖激活 NK-92 细胞产生 TNF-α、IFN-γ、穿孔素、颗粒酶 B、NKG2D 和 FasL。RAW264.7 和 NK-92 细胞的激活是通过 NF-κB 和 MAPKs 信号通路实现的，这表明 NF-κB、ERK、JNK 和 p38 蛋白发生了磷酸化。多糖的结构主要由 →4)-Galp-(1→、→3)-Galp-(1→、→2)-Arap-(1→ 和 →2)-Arap-(1→糖苷键构成。总体结果表明，Mw 较低且构象扩展较大的孜然多糖能更有效地刺激 RAW264.7 和 NK-92 细胞，因此可进一步研究其在生物医学方面的应用。
