Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil.
Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil.
J Ethnopharmacol. 2023 Mar 1;303:115955. doi: 10.1016/j.jep.2022.115955. Epub 2022 Nov 25.
Members of the Psidium genus have been suggested in ethnobotanical research for the treatment of various human diseases, and some studies have already proven their popular uses through research, such as Psidium glaziovianum, which is found in Brazil's northeast and southeast regions and has antinociceptive and anti-inflammatory properties; however, the safety of use has not yet been evaluated.
This study investigated the safety of using essential oil obtained from P. glaziovianum leaves (PgEO) in vitro and in vivo models.
Cytotoxicity was evaluated in murine erythrocytes, while acute toxicity, genotoxicity (comet assay) and mutagenicity (micronucleus test) studies were performed using Swiss albino mice.
In the cytotoxicity assay, the hemolysis rate indicated a low capacity of PgEO to cause cell lysis (0.33-1.78%). In the acute oral toxicity study, animals treated with up to up to 5000 mg/kg body weight did not observe mortality or physiological changes. Neither dosage caused behavioral problems or death in mice over 14 days. The control and 2,000 mg/kg groups had higher feed intake and body weight than the 5,000 mg/kg PgEO group. Erythrocyte count, hemoglobin level, mean corpuscular volume, and MCV decreased, but serum alanine and aspartate aminotransferases increased. In the genotoxic evaluation, 5000 mg/kg PgEO enhanced nucleated blood cell DI and DF.
The present study describes that PgEO can be considered well tolerated in acute exposure at doses up to 2000 mg/kg, however the dose of 5000 mg/kg of PgEO should be used with caution.
Psidium 属的一些成员在民族植物学研究中被用于治疗各种人类疾病，一些研究已经通过研究证明了它们的流行用途，例如在巴西东北部和东南部发现的具有镇痛和抗炎特性的 Psidium glaziovianum；然而，其使用安全性尚未得到评估。
本研究旨在调查从 P. glaziovianum 叶中提取的精油（PgEO）在体外和体内模型中的安全性。
在鼠血红细胞中评估细胞毒性，同时使用瑞士白化小鼠进行急性毒性、遗传毒性（彗星试验）和致突变性（微核试验）研究。
在细胞毒性试验中，溶血率表明 PgEO 引起细胞裂解的能力较低（0.33-1.78%）。在急性口服毒性研究中，用高达 5000mg/kg 体重的 PgEO 处理的动物未观察到死亡率或生理变化。在 14 天内，没有剂量导致老鼠出现行为问题或死亡。对照组和 2000mg/kg 组的摄食量和体重均高于 5000mg/kg PgEO 组。红细胞计数、血红蛋白水平、平均红细胞体积和 MCV 降低，但血清丙氨酸和天冬氨酸转氨酶升高。在遗传毒性评价中，5000mg/kg PgEO 增强了有核血细胞的 DI 和 DF。
本研究表明，PgEO 在急性暴露时，高达 2000mg/kg 的剂量可被认为是耐受良好的，然而，5000mg/kg 的 PgEO 剂量应谨慎使用。
