Costa Wêndeo Kennedy, Guimarães Valquíria Bruna, da Fonsêca Beatriz Meyruze Barros, Ferreira Magda Rhayanny Assunção, Soares Luiz Alberto Lira, Napoleão Thiago Henrique, Paiva Patrícia Maria Guedes, Dos Santos Correia Maria Tereza, Dos Santos Fábio André Brayner, de Oliveira Alisson Macário, da Silva Márcia Vanusa
Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE,, 50670-901, Brazil.
Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil.
Inflammopharmacology. 2024 Feb;32(1):595-602. doi: 10.1007/s10787-023-01351-5. Epub 2023 Oct 12.
Wounds encompass physical, chemical, biological, induced damages to the skin or mucous membranes. In wound treatment, combating infections is a critical challenge due to their potential to impede recovery and inflict systemic harm on patients. Previously, the essential oil extracted from Psidium glaziovianum (PgEO) demonstrated antinociceptive and anti-inflammatory attributes, along with negligible oral toxicity. Hence, our study aimed to assess the effects of topically applying a gel formulation containing PgEO to excisional wounds in mice. Additionally, an in vitro antimicrobial assessment was conducted. The formulated gel underwent characterization and toxicological evaluation on erythrocytes, as well as a dermal irritation test. Its antimicrobial activity was tested against both gram-positive and gram-negative bacteria, as well as fungi. Subsequently, an assessment of its efficacy in excisional wound healing was conducted in mice. The findings of this investigation highlight the gel's efficacy against both gram-positive and gram-negative bacteria, as well as fungi. Moreover, this study underscores that the PgEO-gel treatment enhances skin wound healing, potentially due to its capacity to trigger antioxidant enzymes and suppress pro-inflammatory cytokines. Furthermore, the gel exhibited minimal toxicity to erythrocytes and skin irritation. These findings hold promise for prospective preclinical and clinical trials across diverse wound types. In conclusion, this study sheds light on the potential therapeutic applications of the gel formulation containing essential oil from P. glaziovianum in the context of wound healing.
伤口包括对皮肤或黏膜造成的物理、化学、生物性及人为损伤。在伤口治疗中，对抗感染是一项关键挑战，因为感染有可能阻碍伤口愈合并对患者造成全身性伤害。此前，从番石榴（Psidium glaziovianum）中提取的精油（PgEO）已显示出具有抗伤害感受和抗炎特性，且口服毒性可忽略不计。因此，我们的研究旨在评估局部应用含PgEO的凝胶制剂对小鼠切除伤口的影响。此外，还进行了体外抗菌评估。对所配制的凝胶进行了表征、对红细胞的毒理学评估以及皮肤刺激性试验。测试了其对革兰氏阳性菌、革兰氏阴性菌以及真菌的抗菌活性。随后，在小鼠身上评估了其在切除伤口愈合方面的疗效。本研究结果突出了该凝胶对革兰氏阳性菌、革兰氏阴性菌以及真菌均有效。此外，本研究强调PgEO凝胶治疗可促进皮肤伤口愈合，这可能归因于其触发抗氧化酶和抑制促炎细胞因子的能力。此外，该凝胶对红细胞的毒性和皮肤刺激性极小。这些发现为未来针对各种伤口类型的临床前和临床试验带来了希望。总之，本研究揭示了含番石榴精油的凝胶制剂在伤口愈合方面的潜在治疗应用。
