School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T, 999077, Hong Kong, China.
College of Pharmaceutical Sciences, Hebei University, Baoding, 077000, China.
J Ethnopharmacol. 2023 Mar 1;303:115918. doi: 10.1016/j.jep.2022.115918. Epub 2022 Nov 24.
Fu Rong Ye (FRY), the leaf of Hibiscus mutabilis L., is a Chinese medicinal herb used to treat coughs and respiratory diseases. FRY is the major herbal component of the patent medicine Fupo Ganmao Granules for treating common cold. However, its anti-influenza active components and mechanism were not identified.
Here, we aim to a) isolate the anti-influenza phytochemicals from FRY extract and b) explore its anti-flu mechanism.
Bioassay guided isolation was performed to get anti-influenza virus components. Influenza virus infected cells and mouse model were employed for efficacy evaluation.
Using bioassay-guided isolation, the flavonoid tiliroside was obtained, which inhibited four IAV strains in MDCK cells with EC ranging from 3.87 to 27.61 μM by suppressing the viral ribonucleoprotein activity. Tiliroside also significantly downregulated the expression of cytokines/chemokines in A549 cells, and protected 50% of PR8-infected BALB/c mice from death and at 800 mg/kg/day, improved lung edema conditions.
Tiliroside is effective for influenza virus infection treatment and promising for further drug development. This study is the first to demonstrate that tiliroside in FRY acts against influenza virus.
芙蓉叶（FRY），木槿的叶子，是一种中药，用于治疗咳嗽和呼吸道疾病。FRY 是治疗普通感冒的专利药物复朴感冒颗粒的主要草药成分。然而，其抗流感的活性成分和机制尚未确定。
本研究旨在 a）从 FRY 提取物中分离出抗流感植物化学物质，b）探索其抗流感机制。
采用生物测定指导分离法获得抗流感病毒成分。使用流感病毒感染的细胞和小鼠模型进行功效评估。
通过抑制病毒核糖核蛋白活性，使用生物测定指导分离法获得的类黄酮Tiliroside 抑制了 MDCK 细胞中的四种 IAV 株，EC 范围为 3.87 至 27.61 μM。Tiliroside 还显著下调了 A549 细胞中细胞因子/趋化因子的表达，并使 50%的 PR8 感染的 BALB/c 小鼠免于死亡，在 800mg/kg/天时，改善了肺部水肿状况。
Tiliroside 可有效治疗流感病毒感染，具有进一步开发药物的潜力。本研究首次证明 FRY 中的 Tiliroside 可对抗流感病毒。
