School of Life Sciences, The Chinese University of Hong Kong, Hong Kong 999077, China.
College of Pharmaceutical Sciences, Hebei University, Baoding 077000, China.
Molecules. 2022 Sep 5;27(17):5724. doi: 10.3390/molecules27175724.
Ganlanye (GLY), the leaf of (Lour.) DC., is a traditional Chinese medicinal herb for warm disease treatment. We found that its aqueous extract could inhibit the influenza A virus. To find and characterize anti-influenza virus phytochemicals from GLY, we performed (1) bioassay-guided isolation, (2) a cell and animal assay, and (3) a mechanism study. Bioassay-guided isolation was used to identify the effective components. Influenza virus-infected MDCK cell and BALB/c mouse models were employed to evaluate the anti-influenza virus activities. A MUNANA assay was performed to find the NA inhibitory effect. As a result, urolithin M5 was obtained from the crude extract of GLY. It inhibited influenza virus activities in vitro and in vivo by suppressing the viral NA activity. In the MDCK cell model, urolithin M5 could inhibit an oseltamivir-resistant strain. In a PR8-infected mouse model, 200 mg/kg/d urolithin M5 protected 50% of mice from death and improved lung edema conditions. GLY was recorded as a major traditional herb for warm disease treatment. Our study identified GLY as a potent anti-influenza herb and showed urolithin M5 as the active component. We first report the in vivo activity of urolithin M5 and support the anti-influenza application of GLY.
甘榄叶（GLY），即滇橄榄（Lour.）DC.的叶子，是一种治疗温热病的传统中药。我们发现其水提物可以抑制甲型流感病毒。为了从甘榄叶中寻找并鉴定具有抗流感病毒活性的植物化学物质，我们进行了（1）基于生物测定的分离、（2）细胞和动物检测、（3）机制研究。基于生物测定的分离方法用于鉴定有效成分。我们采用流感病毒感染的 MDCK 细胞和 BALB/c 小鼠模型来评估抗流感病毒活性。采用 MUNANA 法检测神经氨酸酶（NA）抑制作用。结果从甘榄叶粗提取物中得到了尿石素 M5。它通过抑制病毒 NA 活性来抑制病毒在体外和体内的活性。在 MDCK 细胞模型中，尿石素 M5 可以抑制奥司他韦耐药株。在 PR8 感染的小鼠模型中，200mg/kg/d 的尿石素 M5 可使 50%的小鼠免于死亡并改善肺部水肿情况。甘榄叶被记录为一种治疗温热病的主要传统草药。我们的研究鉴定了甘榄叶是一种有效的抗流感草药，并表明尿石素 M5 是其活性成分。我们首次报道了尿石素 M5 的体内活性，并支持甘榄叶在抗流感方面的应用。
