Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, P.R. China.
Aging (Albany NY). 2022 Nov 26;14(22):9186-9199. doi: 10.18632/aging.204400.
Studies suggest that psychological stress on women can impair their reproduction and that postovulatory oocyte aging (POA) might increase the risk of early pregnancy loss and affect offspring's reproductive fitness and longevity. However, whether psychological stress during oocyte development would facilitate POA is unknown but worth exploring to understand the mechanisms by which psychological stress and POA damage oocytes. This study observed effects of female restraint stress during oocyte development (FRSOD) on oocyte resistance to POA. Female mice were restrained for 48 h before superovulation, and they were sacrificed at different intervals after ovulation to recover aging oocytes for analyzing their early and late aged characteristics. The effects of FRSOD on aging oocytes included: (1) increasing their susceptibility to activation stimulus with elevated cytoplasmic calcium; (2) impairing their developmental potential with downregulated expression of development-beneficial genes; (3) facilitating degeneration, cytoplasmic fragmentation and apoptosis; (4) worsening the disorganization of cortical granules and spindle/chromosomes; and (5) impairing redox potential with increased oxidative stress. In conclusion, FRSOD impairs oocyte resistance to POA, so that stressed oocytes become aged significantly quicker than unstressed controls. Thus, couples wishing to achieve pregnancy should take steps to avoid not only fertilization of aged oocytes but also pregestational stressful life events.
研究表明，女性的心理压力会损害其生殖能力，排卵后卵母细胞衰老（POA）可能会增加早期妊娠丢失的风险，并影响后代的生殖健康和寿命。然而，卵母细胞发育过程中的心理压力是否会促进 POA 尚不清楚，但值得探索，以了解心理压力和 POA 损害卵母细胞的机制。本研究观察了卵母细胞发育过程中女性束缚应激（FRSOD）对卵母细胞抵抗 POA 的影响。在超排卵前，雌性小鼠被束缚 48 小时，然后在排卵后不同时间点处死，以恢复老化的卵母细胞，分析其早期和晚期老化特征。FRSOD 对老化卵母细胞的影响包括：（1）增加卵母细胞对激活刺激的敏感性，导致胞质钙离子升高；（2）下调发育有益基因的表达，损害其发育潜能；（3）促进退化、胞质碎片化和凋亡；（4）使皮质颗粒和纺锤体/染色体的排列更加紊乱；（5）增加氧化应激，损害氧化还原电位。总之，FRSOD 损害了卵母细胞抵抗 POA 的能力，使应激卵母细胞比未应激对照卵母细胞衰老得更快。因此，希望怀孕的夫妇应该采取措施，不仅要避免受精老化的卵母细胞，还要避免孕前的应激性生活事件。
