Hendrawan Viski Fitri, Oktanella Yudit, Tuska Habib Syaiful Arif, Al Ichsan Intan Firdha Olien Noor
Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia.
Students of Bachelor's Degree, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia.
Open Vet J. 2024 Nov;14(11):2860-2865. doi: 10.5455/OVJ.2024.v14.i11.14. Epub 2024 Nov 30.
Stress can cause an increase in proinflammatory cytokines, IL-6, which plays a role in the inflammatory response and causes changes in the placenta, causing a low risk of the fetus being born. Giving nanocurcumin, which functions as an anti-inflammatory and antioxidant, is expected to reduce cortisol levels which increase during pregnancy.
This study aims to determine the effect of stress during pregnancy on pregnant mice, namely IL-6 expression and fetal body weight.
This study used 25 mice that were given 5 treatment groups, K(-) with no treatment, K(+) with an induced disturbance of 135 dB for 40 minutes with a continuous pattern, P1, P2, and P3 were given a disturbance of 135 dB for 40 minutes. With a continuous pattern and nanocurcumin doses of 14 mg/kg BW, 21 mg/kgBW, and 24.5 mg/kgBW. IL-6 expression in placental organs was measured using immunohistochemistry and fetal body weight was weighed using a digital balance. Data were analyzed using the Kruskal Wallis test and continued with the Parwise Comparison test.
The results of analysis of IL-6 expression in the placenta showed no significant difference in the P3 group compared to the negative control group. The results of fetal weight analysis showed no significant difference in groups P2 and P3 with the negative control group.
The conclusion of this study is the administration of nanocurcumin in group P3 has been proven to increase IL-6 to a level comparable to the negative control group ( < 0.05). Similarly, the administration of nanocurcumin in groups P2 and P3 can increase fetal weight to a level comparable to the negative control group ( < 0.05).
压力会导致促炎细胞因子白细胞介素-6（IL-6）增加，其在炎症反应中起作用，并导致胎盘发生变化，致使胎儿出生风险降低。给予具有抗炎和抗氧化作用的纳米姜黄素，有望降低孕期升高的皮质醇水平。
本研究旨在确定孕期应激对孕鼠的影响，即IL-6表达和胎儿体重。
本研究使用25只小鼠，分为5个治疗组，K（-）组不进行处理，K（+）组以连续模式给予135分贝的诱导干扰40分钟，P1、P2和P3组给予135分贝的干扰40分钟，连续模式且纳米姜黄素剂量分别为14毫克/千克体重、21毫克/千克体重和24.5毫克/千克体重。使用免疫组织化学法测量胎盘器官中的IL-6表达，使用电子天平称量胎儿体重。数据采用Kruskal Wallis检验进行分析，随后进行两两比较检验。
胎盘IL-6表达分析结果显示，P3组与阴性对照组相比无显著差异。胎儿体重分析结果显示，P2组和P3组与阴性对照组无显著差异。
本研究的结论是，P3组给予纳米姜黄素已被证明可使IL-6增加至与阴性对照组相当的水平（<0.05）。同样，P2组和P3组给予纳米姜黄素可使胎儿体重增加至与阴性对照组相当的水平（<0.05）。
