Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084, PR China.
Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084, PR China; Beijing Key Laboratory of Radioactive Waste Treatment, INET, Tsinghua University, Beijing 100084, PR China.
Bioresour Technol. 2023 Feb;369:128401. doi: 10.1016/j.biortech.2022.128401. Epub 2022 Nov 26.
The effect of ferric iron (Fe(Ш)) on the performance of heterotrophic solid-phase denitrification (SPD) using biodegradable polymer composite as the electron donor was investigated. The results of continuous batch experiments showed that the addition of over 10 mg/L Fe(Ш) significantly inhibited nitrate removal and led to the accumulation of nitrite. The addition of Fe(Ш) reduced the microbial community diversity and shifted the community dominated by complete denitrifiers (e.g. Thauera) to that dominated by incomplete denitrifiers (e.g. Thermomonas, Stenotrophomonas and Sphingomonas). The predicted analysis of microbial function by PICRUSt2 indicated that the relative abundance of denitrifying genes, including napA/B, nirS and nosZ, were remarkably reduced in the Fe(Ш) groups comparing with the control group. In addition, Fe(Ш) inhibited the genes related to the generation of electron carriers, NADH and FADH, in TCA cycle and glycolysis processes, which could result in a lower carbon utilization efficiency for microbial denitrification.
研究了三价铁（Fe(Ш)）对可生物降解聚合物复合材料作为电子供体的异养固相反硝化（SPD）性能的影响。连续批处理实验结果表明，添加超过 10mg/L 的 Fe(Ш)会显著抑制硝酸盐去除并导致亚硝酸盐积累。Fe(Ш)的添加降低了微生物群落的多样性，并将由完全反硝化菌（如 Thauera）主导的群落转变为由不完全反硝化菌（如 Thermomonas、 Stenotrophomonas 和 Sphingomonas）主导的群落。通过 PICRUSt2 对微生物功能的预测分析表明，与对照组相比，Fe(Ш)组中反硝化基因（包括 napA/B、nirS 和 nosZ）的相对丰度显著降低。此外，Fe(Ш)抑制了 TCA 循环和糖酵解过程中与电子载体 NADH 和 FADH 生成相关的基因，这可能导致微生物反硝化的碳利用效率降低。
