Suppr超能文献

基因组编辑

Genome editing.

作者信息

McGrail Maura, Sakuma Tetsushi, Bleris Leonidas

机构信息

Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.

Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan.

出版信息

Sci Rep. 2022 Nov 28;12(1):20497. doi: 10.1038/s41598-022-24850-x.

DOI:10.1038/s41598-022-24850-x
PMID:36443399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9705536/
Abstract

Recent advances in genome editing technologies have redefined our ability to probe and precisely edit the human genome and epigenome in vitro and in vivo More specifically, RNA-guided CRISPR/Cas systems have revolutionized the field due to their simplicity in design and adaptability across biological systems. This Collection highlights results in CRISPR/Cas technology that increase the efficiency of precision genome editing, and allow genetic manipulation in model systems traditionally intractable to site-directed gene modification.

摘要

基因组编辑技术的最新进展重新定义了我们在体外和体内探测及精确编辑人类基因组和表观基因组的能力。更具体地说，RNA引导的CRISPR/Cas系统因其设计简单且能在生物系统中广泛应用，给该领域带来了变革。本合集重点介绍了CRISPR/Cas技术的相关成果，这些成果提高了精确基因组编辑的效率，并能在传统上难以进行定点基因修饰的模型系统中实现基因操作。

相似文献

1
Genome editing.基因组编辑
Sci Rep. 2022 Nov 28;12(1):20497. doi: 10.1038/s41598-022-24850-x.
2
[Preface for genome editing special issue].[基因组编辑特刊前言]
Sheng Wu Gong Cheng Xue Bao. 2017 Oct 25;33(10):1661-1664. doi: 10.13345/j.cjb.170374.
3
Human Genome Editing and Ethical Considerations.人类基因组编辑与伦理考量。
Sci Eng Ethics. 2016 Apr;22(2):597-9. doi: 10.1007/s11948-015-9675-8. Epub 2015 Jul 8.
4
[Genome editing ~Principle and possibility of a novel genetic engineering technology. Basic principles of genome editing.].[基因组编辑~一种新型基因工程技术的原理与可能性。基因组编辑的基本原理。]
Clin Calcium. 2017;27(11):1638-1644.
5
[Basics and applications of genome editing technology].[基因组编辑技术的基础与应用]
Rinsho Ketsueki. 2016;57(10):1869-1873. doi: 10.11406/rinketsu.57.1869.
6
Prime Editing: Precision Genome Editing by Reverse Transcription.碱基编辑：基于逆转录的精确基因组编辑技术
Mol Cell. 2020 Jan 16;77(2):210-212. doi: 10.1016/j.molcel.2019.12.016.
7
[Genome editing technology and its application in forage legumes].[基因组编辑技术及其在豆科牧草中的应用]
Sheng Wu Gong Cheng Xue Bao. 2017 Oct 25;33(10):1733-1743. doi: 10.13345/j.cjb.170169.
8
[Application of genome editing technology in gene therapy].[基因组编辑技术在基因治疗中的应用]
Rinsho Ketsueki. 2022;63(11):1558-1565. doi: 10.11406/rinketsu.63.1558.
9
Molecular Switch Engineering for Precise Genome Editing.分子开关工程用于精确基因组编辑。
Bioconjug Chem. 2021 Apr 21;32(4):639-648. doi: 10.1021/acs.bioconjchem.1c00088. Epub 2021 Apr 7.
10
Therapeutic application of sequence-specific binding molecules for novel genome editing tools.序列特异性结合分子在新型基因组编辑工具中的治疗应用。
Drug Metab Pharmacokinet. 2022 Feb;42:100427. doi: 10.1016/j.dmpk.2021.100427. Epub 2021 Oct 24.

引用本文的文献

1
The Tetracycline-Inducible/CRISPR-Cas9 System is an Efficient Tool for Studying Gene Function in Phytophthora sojae.四环素诱导型/CRISPR-Cas9系统是研究大豆疫霉基因功能的有效工具。
Mol Plant Pathol. 2025 Jun;26(6):e70114. doi: 10.1111/mpp.70114.
2
Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders.利用体内电穿孔技术研究脑发育障碍的分子机制的高级技术。
Int J Mol Sci. 2023 Sep 15;24(18):14128. doi: 10.3390/ijms241814128.

本文引用的文献

1
Dual-sgRNA CRISPR/Cas9 knockout of PD-L1 in human U87 glioblastoma tumor cells inhibits proliferation, invasion, and tumor-associated macrophage polarization.CRISPR/Cas9 双 sgRNA 敲除人 U87 神经胶质瘤肿瘤细胞中的 PD-L1 抑制增殖、侵袭和肿瘤相关巨噬细胞极化。
Sci Rep. 2022 Feb 14;12(1):2417. doi: 10.1038/s41598-022-06430-1.
2
Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking.通过串联配对切口实现高效靶向基因敲入的实验策略。
Sci Rep. 2021 Nov 19;11(1):22627. doi: 10.1038/s41598-021-01978-w.
3
A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines.一种可选择的一体式 CRISPR 先导编辑 piggyBac 转座子，可实现人细胞系中的高效基因编辑。
Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2.
4
Cas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells.Cas9 介导的基因组编辑揭示了钙信号通路对 Pv11 细胞脱水休眠的重要贡献。
Sci Rep. 2021 Oct 5;11(1):19698. doi: 10.1038/s41598-021-98905-w.
5
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair.CRISPR Cas9 和 Cas12a 同源定向修复的优化设计参数。
Sci Rep. 2021 Sep 30;11(1):19482. doi: 10.1038/s41598-021-98965-y.
6
CRISPR/Cas9 small promoter deletion in H19 lncRNA is associated with altered cell morphology and proliferation.CRISPR/Cas9 对 H19 lncRNA 小启动子缺失与细胞形态和增殖改变有关。
Sci Rep. 2021 Sep 15;11(1):18380. doi: 10.1038/s41598-021-97058-0.
7
Duchenne muscular dystrophy cell culture models created by CRISPR/Cas9 gene editing and their application in drug screening.CRISPR/Cas9 基因编辑构建的杜氏肌营养不良症细胞培养模型及其在药物筛选中的应用。
Sci Rep. 2021 Sep 14;11(1):18188. doi: 10.1038/s41598-021-97730-5.
8
Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA polymerase θ/DNA-PK.通过供体体内线性化和瞬时抑制 DNA 聚合酶 θ/DNA-PK 实现小鼠胚胎干细胞的高效双等位基因敲入。
Sci Rep. 2021 Sep 13;11(1):18132. doi: 10.1038/s41598-021-97579-8.
9
Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants.靶向敲除基因 OsHOL1 可消除水稻植株的甲碘排放。
Sci Rep. 2021 Aug 23;11(1):17010. doi: 10.1038/s41598-021-95198-x.
10
Investigating crosstalk between H3K27 acetylation and H3K4 trimethylation in CRISPR/dCas-based epigenome editing and gene activation.研究基于 CRISPR/dCas 的表观基因组编辑和基因激活中 H3K27 乙酰化与 H3K4 三甲基化之间的串扰。
Sci Rep. 2021 Aug 5;11(1):15912. doi: 10.1038/s41598-021-95398-5.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验