College of Bioscience and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China.
Hangzhou Medical College, Hangzhou 310053, Zhejiang, China.
Food Chem. 2023 Mar 15;404(Pt A):134464. doi: 10.1016/j.foodchem.2022.134464. Epub 2022 Sep 30.
A simple, rapid and sensitive vortex-assisted magnetic solid phase extraction (MSPE) method using core-shell structured magnetic covalent organic frameworks (FeO/COF-TpBD) as adsorbents was established for simultaneous determination of aflatoxins, ochratoxins and enniatins in maize before high-performance chromatography tandem spectrometry detection. FeO/COF-TpBD was facilely fabricated, characterized, and applied for simultaneous extraction of ten mycotoxins from maize. Key parameters affecting MSPE efficiency were investigated. The method possessed good linearity (0.05-50 μg/kg), high sensitivity (0.02-1.67 µg/kg for limits of detection), and satisfactory recoveries (73.8-105.3 %), with relative standard deviations less than 8.5 %. The amount of adsorbent used (5 mg) and the time required (0.5 min each for adsorption and for desorption) for MSPE were greatly shortened in comparison with the previous reports. Therefore, the fabricated FeO/COF-TpBD exhibited a great potential for rapid extraction of common mycotoxins in maize, which can be attributed to its multiple interactions with the target mycotoxins.
建立了一种简便、快速、灵敏的基于磁固相萃取（MSPE）的方法，采用核壳结构的磁性共价有机框架（FeO/COF-TpBD）作为吸附剂，用于高效液相色谱串联质谱法检测前对玉米中黄曲霉毒素、赭曲霉毒素和恩镰孢菌素的同时测定。FeO/COF-TpBD 易于制备、表征，并用于从玉米中同时提取十种真菌毒素。考察了影响 MSPE 效率的关键参数。该方法具有良好的线性（0.05-50μg/kg）、高灵敏度（0.02-1.67μg/kg，检出限）和令人满意的回收率（73.8-105.3%），相对标准偏差小于 8.5%。与以前的报道相比，MSPE 中吸附剂的用量（5mg）和洗脱时间（吸附和洗脱各 0.5min）大大缩短。因此，所制备的 FeO/COF-TpBD 对玉米中常见真菌毒素的快速提取具有很大的潜力，这归因于其与目标真菌毒素的多种相互作用。
