三叶鬼针草在体外对人肝癌 HepG2 细胞具有抗增殖和促凋亡作用。
Sida rhombifolia Exerts Anti-Proliferative and Pro-Apoptotic Effects in Human Liver Cancer HepG2 Cells in Vitro.
机构信息
Molecular and Cell Biology Research Center, Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Biotechnology, Payame Noor University, Tehran, Iran.
出版信息
Asian Pac J Cancer Prev. 2022 Nov 1;23(11):3677-3684. doi: 10.31557/APJCP.2022.23.11.3677.
PURPOSE
Modern research revealed that plants belonging to the Sida rhombifolia family (Malvaceae) contain biologically active compounds that make them prone to discovering and developing anticancer drugs. This study aimed to evaluate the apoptosis effects of S. rhombifolia extracts in HepG2 Cell Line was performed.
METHODS
The extractions were prepared, and an MTT assay was applied to evaluate its role in decreasing the viability of HepG2 and HFF cells. Phenolic compounds were analyzed using High-performance liquid chromatography (HPLC). FlowCytometry and RT-qPCR evaluated apoptosis was performed to measure the mRNA expression of pro-and anti-apoptotic mediators.
RESULTS
The results can be summarized as EtOAc extract was more cytotoxic against the HepG2 cells (IC50= 364.3 µg/mL) compared to MeOH and HEX extracts (720.2 µg/mL) (560.4 µg/mL) with less cytotoxicity in HFF cells (353.2 µg/mL). The HPLC analysis results revealed most phenolic compounds, such as Epicatechin(1.3 mg/g). The EtOAc extract (300 μg/mL) induced 34% apoptosis in HepG2 cells. RT-qPCR data showed upregulation of the proapoptotic gene (Bax) and increased Bax/BCL-2 ratio by S. rhombifolia EtOAc extract (300 μg/mL).
CONCLUSION
In conclusion, the EtOAc extract of S. rhombifolia is capable of inducing apoptosis in HepG2 cells through modulation of the mitochondrial pathway, which explains their antitumor activity.
目的
现代研究表明，属于苘麻科（锦葵科）的植物含有具有生物活性的化合物，使它们易于发现和开发抗癌药物。本研究旨在评估 Sida rhombifolia 提取物对 HepG2 细胞系的凋亡作用。
方法
制备提取物，应用 MTT assay 评估其降低 HepG2 和 HFF 细胞活力的作用。采用高效液相色谱法（HPLC）分析酚类化合物。流式细胞术和 RT-qPCR 评估凋亡，以测量促凋亡和抗凋亡介质的 mRNA 表达。
结果
结果可概括为：EtOAc 提取物对 HepG2 细胞的细胞毒性（IC50=364.3 µg/mL）高于 MeOH 和 HEX 提取物（720.2 µg/mL）（560.4 µg/mL），对 HFF 细胞的细胞毒性较小（353.2 µg/mL）。HPLC 分析结果显示，大多数酚类化合物，如表儿茶素（1.3 mg/g）。EtOAc 提取物（300 µg/mL）诱导 HepG2 细胞凋亡 34%。RT-qPCR 数据显示，S. rhombifolia EtOAc 提取物（300 µg/mL）上调促凋亡基因（Bax），并增加 Bax/BCL-2 比值。
结论
总之，S. rhombifolia 的 EtOAc 提取物能够通过调节线粒体途径诱导 HepG2 细胞凋亡，这解释了其抗肿瘤活性。
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