Department of General Surgery, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China.
Anesthesiology Department, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China.
J Clin Lab Anal. 2022 Dec;36(12):e24790. doi: 10.1002/jcla.24790. Epub 2022 Nov 29.
Chitinase 3-Like 1 (CHI3L1) has been used as an inflammatory biomarker for a variety of diseases, but its expression in acute appendicitis and appendix carcinomas remains unclear.
Sixty cases of patients were studied, including 46 acute appendicitis and 14 appendix carcinomas. We divided the acute appendicitis group into acute uncomplicated appendicitis (AUA), suppurative appendicitis (SA), and gangrenous appendicitis (GA). The appendix carcinoma group was divided into appendiceal neuroendocrine neoplasms (ANENs) and appendiceal mucinous neoplasms (AMN). Controls were 32 healthy donors. Blood neutrophil to lymphocyte ratio (NLR), CHI3L1, C-reactive protein (CRP), interleukin-6 (IL-6), and serum amyloid A (SAA) were measured in the patients. Meanwhile, immunohistochemistry and immunofluorescence were used to identify the expression level and location of CHI3L1 in different cell types in appendix tissues.
Compared with the controls, CHI3L1 serum levels were up-regulated in SA, GA, and AMN groups, while no significant difference was observed in the AUA and ANEN groups. Immunofluorescence revealed that CHI3L1 expression was high in macrophages and adenocarcinoma cells of appendix tissues but not in the neuroendocrine carcinoma tissues. Moreover, levels of NLR and CRP in the SA and GA groups were considerably higher than in the control group. IL-6 and SAA in SA, GA, ANENs, and AMN groups were also increased compared with the control group. In addition, CHI3L1 displayed good performance in predicting appendicitis, with an AUC of 0.862.
CHI3L1 was highly expressed in acute appendicitis and appendiceal mucinous neoplasms, which can be used as a novel biomarker predicting appendicitis.
几丁质酶 3 样蛋白 1（CHI3L1）已被用作多种疾病的炎症生物标志物，但在急性阑尾炎和阑尾癌中的表达尚不清楚。
研究了 60 例患者，包括 46 例急性阑尾炎和 14 例阑尾癌。我们将急性阑尾炎组分为急性单纯性阑尾炎（AUA）、化脓性阑尾炎（SA）和坏疽性阑尾炎（GA）。阑尾癌组分为阑尾神经内分泌肿瘤（ANENs）和阑尾黏液性肿瘤（AMN）。对照组为 32 名健康供者。测量了患者的血液中性粒细胞与淋巴细胞比值（NLR）、CHI3L1、C 反应蛋白（CRP）、白细胞介素 6（IL-6）和血清淀粉样蛋白 A（SAA）。同时，采用免疫组织化学和免疫荧光法检测 CHI3L1 在阑尾组织不同细胞类型中的表达水平和位置。
与对照组相比，SA、GA 和 AMN 组的血清 CHI3L1 水平升高，而 AUA 和 ANEN 组无显著差异。免疫荧光显示 CHI3L1 在阑尾组织中的巨噬细胞和腺癌细胞中高表达，但在神经内分泌癌组织中不表达。此外，SA 和 GA 组的 NLR 和 CRP 水平明显高于对照组。SA、GA、ANENs 和 AMN 组的 IL-6 和 SAA 也高于对照组。此外，CHI3L1 在预测阑尾炎方面表现出良好的性能，AUC 为 0.862。
CHI3L1 在急性阑尾炎和阑尾黏液性肿瘤中高表达，可作为预测阑尾炎的新型生物标志物。
