Oguntoye Modupeola A, Oridupa Olayinka A, Ezekiel Olufunke O
Department of Food Technology, University of Ibadan, Ibadan, Nigeria.
Department of Veterinary Pharmacology and Toxicology, University of Ibadan, Ibadan, Nigeria.
J Food Sci. 2023 Jan;88(1):491-502. doi: 10.1111/1750-3841.16391. Epub 2022 Nov 30.
In vitro antioxidant activity and possible in vivo antioxidative effects of provitamin A cassava hydrolysate inoculated with free or encapsulated Lactobacillus rhamnosus GG (LGG), a ready-to-drink probiotic beverage containing natural β-carotene and other phytochemicals, were evaluated. Provitamin A cassava was orally administered to male Wistar rats (n = 40, 120-150 g) for 30 days. Regression analysis showed the phenolic and total flavonoid contents of provitamin A cassava hydrolysate contributed 68% and 95%, respectively, to the antioxidant activity (by 1,1-diphenyl-2-picrylhydrazyl assay) (83.3 ± 1.8-87 ± 1.8%). in vivo study showed that malondialdehyde and superoxide dismutase activities were lowered or unchanged with a significant increase in antioxidant biomarkers (reduced glutathione, glutathione-S-transferase, and glutathione peroxidase) in the kidney, heart, and liver of all rats administered provitamin A cassava hydrolysate with LGG, especially at doses 2 × 10 CFU, compared to control. Provitamin A cassava hydrolysate with LGG showed significant antioxidant activity In vitro and in vivo, thus indicating its potential usefulness in the food industry as an antioxidant beverage. PRACTICAL APPLICATION: This study reports the antioxidant properties of provitamin A cassava hydrolysate, which can be consumed as a refreshing beverage while also delivering antioxidant benefits. In the food and beverage industry, provitamin A cassava hydrolysate could be applied as a nutraceutical beverage with therapeutic functions. This product innovation increases the value-addition of cassava and expands the nondairy food matrix options used as probiotic carriers.
对接种游离或包封的鼠李糖乳杆菌GG（LGG）的维生素A原木薯水解物的体外抗氧化活性及可能的体内抗氧化作用进行了评估，该水解物是一种即饮型益生菌饮料，含有天然β-胡萝卜素和其他植物化学物质。将维生素A原木薯口服给予雄性Wistar大鼠（n = 40，体重120 - 150克），持续30天。回归分析表明，维生素A原木薯水解物中的酚类和总黄酮含量分别对其抗氧化活性（通过1,1 - 二苯基 - 2 - 苦基肼法测定）贡献了68%和95%（抗氧化活性为83.3 ± 1.8 - 87 ± 1.8%）。体内研究表明，与对照组相比，所有给予含LGG的维生素A原木薯水解物的大鼠，其肾脏、心脏和肝脏中的丙二醛和超氧化物歧化酶活性降低或未改变，而抗氧化生物标志物（还原型谷胱甘肽、谷胱甘肽 - S - 转移酶和谷胱甘肽过氧化物酶）显著增加，尤其是在剂量为2×10 CFU时。含LGG的维生素A原木薯水解物在体外和体内均表现出显著的抗氧化活性，因此表明其在食品工业中作为抗氧化饮料具有潜在用途。实际应用：本研究报道了维生素A原木薯水解物的抗氧化特性，它既可以作为提神饮料饮用，又能提供抗氧化益处。在食品和饮料行业，维生素A原木薯水解物可作为具有治疗功能的营养饮料应用。这种产品创新增加了木薯的附加值，并扩展了用作益生菌载体的非乳制品食品基质选择。
