Department of Physiology, Sakarya University, Medical Faculty, Sakarya, Turkey.
Department of Medical Biology, Medical Faculty, Kutahya Health Sciences University, Kutahya, Turkey.
J Sci Food Agric. 2024 Oct;104(13):8109-8119. doi: 10.1002/jsfa.13641. Epub 2024 Jun 10.
Stress-related diseases are on the rise and stress is one of the common factors that lead to ulcer. Stress-induced mucosal bleeding is a serious complication observed in many critically ill patients. Due to the harmful side effects of proton pump inhibitors, natural and active alternative treatment methods for peptic ulcer treatment that are safe in terms of side effects are an urgent need for human health. We aimed to investigate the dose-dependent protective effects of Lactobacillus rhamnosus GG (LGG) against stress ulcer induced by cold restraint stress in rats. This study was performed in a total of 42 rats, in control group (C), stress group (S), pantoprazole (20 mg kg day) group (P), LGG (3 × 10 cfu mL day) + stress group (M1), LGG (15 × 10 mL day) + stress group (M5) and LGG (30 × 10 mL day) + stress group (M10) (each n = 7). Ulceration areas (mm) were determined quantitatively with ImageJ software. Glucocorticoid, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels were determined by ELISA and malondialdehyde levels were determined by spectrophotometric measurement. Histopathological examinations were performed in gastric tissue.
Therapeutic dose of LGG increased CAT, SOD and GPx levels; prevented excessive activation of the hypothalamic-pituitary-adrenal axis; reduced ulceration and bleeding in the gastric mucosal layer; and provided stabilization of mast cells.
We can suggest that LGG may be beneficial for reducing the negative effects of stress on the body, for protecting against ulcer disease and for reducing or preventing the risk of stress-induced gastrointestinal bleeding in patients staying in intensive care units. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
与压力相关的疾病呈上升趋势，而压力是导致溃疡的常见因素之一。应激诱导的黏膜出血是许多危重病患者中观察到的严重并发症。由于质子泵抑制剂的有害副作用，人们迫切需要寻找一种安全有效的替代治疗方法来治疗消化性溃疡。本研究旨在探讨鼠李糖乳杆菌 GG（LGG）对冷束缚应激诱导的大鼠应激性溃疡的剂量依赖性保护作用。本研究共纳入 42 只大鼠，分为对照组（C）、应激组（S）、泮托拉唑（20mg/kg/天）组（P）、LGG（3×10cfu/mL/天）+应激组（M1）、LGG（15×10cfu/mL/天）+应激组（M5）和 LGG（30×10cfu/mL/天）+应激组（M10）（每组 n=7）。采用 ImageJ 软件定量测定溃疡面积（mm）。采用 ELISA 法测定糖皮质激素、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）水平，采用分光光度法测定丙二醛（MDA）水平。对胃组织进行组织病理学检查。
治疗剂量的 LGG 增加了 CAT、SOD 和 GPx 水平；防止了下丘脑-垂体-肾上腺轴的过度激活；减少了胃黏膜层的溃疡和出血；并稳定了肥大细胞。
我们可以认为 LGG 可能有益于减轻压力对身体的负面影响，预防溃疡病，并降低或预防 ICU 患者应激性胃肠道出血的风险。© 2024 作者。《食品科学杂志》由 John Wiley & Sons Ltd 代表化学工业协会出版。
