BioBank, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 710061, China.
Department of Psychiatry, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 710061, China.
Cell Rep Methods. 2022 Nov 4;2(11):100324. doi: 10.1016/j.crmeth.2022.100324. eCollection 2022 Nov 21.
The gut microbiome is essential for human health. Mouse microbiota models, including gnotobiotic mice, are the most prominent tools to elucidate the functions of gut bacteria. Here, we propose a targeted-bacterium-depleted (TBD) model using lytic bacteriophage to selectively deplete gut bacterium of healthy or otherwise defined mice. These phage-treated animals should have a near-complete spectrum of gut bacteria except for the depleted bacterium. To prove the concept, we employed -specific phage T7 to repress in the healthy mice. Our results showed that the depleted mice exhibited bravery-like behaviors, correlated to the presence of rather than the equilibrium among gut bacteria. Thus, we demonstrate that the TBD model is a powerful tool to elucidate the function of a specific bacterial species within a near-intact gut microbiota environment and complements gnotobiotic mice models.
肠道微生物组对人类健康至关重要。包括无菌小鼠在内的鼠类微生物组模型是阐明肠道细菌功能的最主要工具。在这里，我们提出了一种使用裂解噬菌体的靶向细菌耗竭（TBD）模型，以选择性地耗竭健康或其他定义的小鼠的肠道细菌。这些经噬菌体处理的动物除了耗竭的细菌外，应该具有近乎完整的肠道细菌谱。为了证明这一概念，我们使用特异性噬菌体 T7 来抑制健康小鼠中的 。我们的结果表明，耗竭的小鼠表现出勇敢样行为，这与 的存在相关，而不是与肠道细菌的平衡相关。因此，我们证明了 TBD 模型是一种强大的工具，可以在近乎完整的肠道微生物群环境中阐明特定细菌物种的功能，并补充无菌小鼠模型。
