Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.
Physiol Plant. 2022 Nov;174(6):e13836. doi: 10.1111/ppl.13836.
The bean fruit pericarp accumulates a significant amount of starch, which starts to be degraded 20 days after anthesis (DAA) when seed growth becomes exponential. This period is also characterized by the progressive senescence of the fruit pericarp. However, the chloroplasts maintained their integrity, indicating that starch degradation is a compartmentalized process. The process coincided with a transient increase in maltose and sucrose levels, suggesting that β-amylase is responsible for starch degradation. Starch degradation in the bean fruit pericarp is also characterized by a large increase in starch phosphorylation, as well as in the activities of cytosolic disproportionating enzyme 2 (DPE2, EC 2.4.1.25) and glucan phosphorylase (PHO2, EC 2.4.1.1). This suggests that the rate of starch degradation in the bean fruit pericarp 20 DAA is dependent on the transformation of starch to a better substrate for β-amylase and the increase in the rate of cytosolic metabolism of maltose.
豆科果实的果皮中积累了大量的淀粉,在授粉后 20 天(DAA)种子生长呈指数增长时,淀粉开始降解。这一时期也是果实果皮逐渐衰老的特征。然而,叶绿体保持完整,表明淀粉降解是一个分隔的过程。这一过程伴随着麦芽糖和蔗糖水平的短暂增加,表明β-淀粉酶负责淀粉的降解。豆科果实果皮中淀粉的降解还伴随着淀粉磷酸化的大量增加,以及胞质歧化酶 2(DPE2,EC 2.4.1.25)和葡聚糖磷酸化酶(PHO2,EC 2.4.1.1)活性的增加。这表明,20 DAA 时豆科果实果皮中淀粉的降解速度依赖于淀粉向更适合β-淀粉酶的底物的转化,以及胞质麦芽糖代谢速度的增加。