Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan.
Advanced Research Facilities and Services, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan.
Forensic Toxicol. 2022 Jan;40(1):189-198. doi: 10.1007/s11419-021-00594-7. Epub 2021 Sep 16.
The quantification of parent molecules of pyrethroids tetramethrin and resmethrin in human specimens by a mass spectrometry (MS) technique has not been reported yet. A woman in her 60s was found dead in a wasteland. At the scene, an empty beer can and a spray for insecticides containing tetramethrin and resmethrin were found. Therefore, the concentrations of tetramethrin and resmethrin in postmortem specimens and the methanol solution used for rinsing the inside of the beer can were determined using liquid chromatography (LC)-tandem mass spectrometry (MS/MS).
The quantification method by LC-MS/MS for intact parent molecules of tetramethrin and resmethrin in whole blood and urine has been devised and validated in this work. The method was applied to the quantification of tetramethrin and resmethrin in whole blood, urine and stomach contents obtained from a cadaver at autopsy.
The limits of detection of tetramethrin and resmethrin were 0.06 and 0.03 ng/mL; limits of quantification were 0.2 and 0.1 ng/mL in blood and urine, respectively. The concentrations of tetramethrin of the deceased were 11.1 ± 1.2 and 0.425 ± 0.017 ng/mL for stomach contents and urine, respectively; the concentration of resmethrin in stomach contents was 1.77 ± 0.18 ng/mL. The tetramethrin and resmethrin were unstable in blood and urine at room temperature; they should be kept at not higher than 4 ℃.
To our knowledge, this is the first report for quantification of unchanged tetramethrin and resmethrin in human specimens obtained in a fatal case.
目前尚未有报道采用质谱（MS）技术定量检测拟除虫菊酯四甲菊酯和雷沙米林母体分子在人体标本中的含量。一位 60 多岁的女性被发现在荒地中死亡。在现场发现了一个空啤酒罐和一个含有四甲菊酯和雷沙米林的杀虫剂喷雾器。因此，使用液相色谱（LC）-串联质谱（MS/MS）法测定了死后标本和用于冲洗啤酒罐内部的甲醇溶液中四甲菊酯和雷沙米林的浓度。
本工作设计并验证了 LC-MS/MS 法用于检测全血和尿液中完整的四甲菊酯和雷沙米林母体分子。该方法应用于定量分析尸检获得的全血、尿液和胃内容物中的四甲菊酯和雷沙米林。
四甲菊酯和雷沙米林的检测限分别为 0.06 和 0.03 ng/mL；定量限分别为 0.2 和 0.1 ng/mL，用于血液和尿液。死者胃内容物和尿液中四甲菊酯浓度分别为 11.1±1.2 和 0.425±0.017 ng/mL；胃内容物中雷沙米林浓度为 1.77±0.18 ng/mL。四甲菊酯和雷沙米林在室温下于血液和尿液中不稳定；应保持在 4℃以下。
据我们所知，这是首例报道在致命案例中定量检测人标本中未改变的四甲菊酯和雷沙米林。
