Tabasum Husna, Neelagund S E, Kotresh K R, Gowtham M D, Sulochana N
Department of Biochemistry, Jnanasahyadri, Kuvempu University, Shankaraghatta, Shimoga, Karnataka, 577451, India; State Forensic Science Laboratory, Madivala, Bengaluru, Karnataka, 560068, India.
Department of Biochemistry, Jnanasahyadri, Kuvempu University, Shankaraghatta, Shimoga, Karnataka, 577451, India.
J Forensic Leg Med. 2022 Oct;91:102423. doi: 10.1016/j.jflm.2022.102423. Epub 2022 Aug 12.
The ingestion of the insecticide chlorpyrifos leads to fatal intoxication in suicidal cases, and its distribution can be assessed only after post-mortem. This study attempted to investigate the distribution of chlorpyrifos in forensic visceral tissue samples like stomach, liver, kidney, heart, brain, lung, spleen, muscle and body fluids like blood and urine by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with the liquid-liquid extraction method. An analysis time of 8.77 min with 1 μl as an injection volume was chosen. The results obtained are analysed using Labsolutions insight LCMS software, considering Multiple Reaction Monitoring (MRM) and Retention Time (RT). Our findings depict that the stomach tissue has very high concentrations of chlorpyrifos, which suggests the possibility of high distribution and aspiration in the stomach tissue. The concentration of chlorpyrifos in stomach tissue ranged from 816 to 901 μg/g in male cadavers and from 443 to 612 μg/g in female cadavers. Among all the tissues, the stomach tissue showed the highest concentration, while the lowest concentration was found in muscle. Metabolic distribution of chlorpyrifos from the stomach contents to the other surrounding organs may occur and hence the concentrations of CPF were also seen in other organs like the liver, lung, kidney, brain, and muscle. These toxicological results from autopsy findings, together with LC-MS/MS indicate that stomach tissue examination gives an accurate profile of insecticide poisoning in forensic samples with acute poisoning.
摄入杀虫剂毒死蜱在自杀案例中会导致致命中毒，且只有在死后才能评估其分布情况。本研究试图通过液相色谱-串联质谱法（LC-MS/MS）结合液-液萃取法，调查毒死蜱在法医内脏组织样本（如胃、肝、肾、心、脑、肺、脾、肌肉）以及血液和尿液等体液中的分布。选择进样体积为1μl时分析时间为8.77分钟。使用Labsolutions insight LCMS软件，考虑多反应监测（MRM）和保留时间（RT）对所得结果进行分析。我们的研究结果表明，胃组织中毒死蜱浓度非常高，这表明胃组织中存在高分布和吸入的可能性。男性尸体胃组织中毒死蜱浓度范围为816至901μg/g，女性尸体为443至612μg/g。在所有组织中，胃组织浓度最高，而肌肉中浓度最低。毒死蜱可能会从胃内容物代谢分布到其他周围器官，因此在肝脏、肺、肾、脑和肌肉等其他器官中也检测到了毒死蜱的浓度。尸检结果的这些毒理学结果与LC-MS/MS一起表明，胃组织检查能准确显示法医样本中急性中毒的杀虫剂中毒情况。
