Laboratório de Polímeros e Inovação de Materiais, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Fortaleza, Brazil.
Centro de Estudos Farmacêuticos e Cosméticos, Departamento de Farmácia, Universidade Federal do Ceará, Fortaleza, Brazil.
J Biomed Mater Res B Appl Biomater. 2023 May;111(5):1035-1047. doi: 10.1002/jbm.b.35212. Epub 2022 Dec 1.
This work aimed the development and evaluation of the wound healing activity of films based on sodium alginate, polyvinyl alcohol (PVA) and Ca loaded with Agaricus blazei Murill hydroalcoholic extract (AbE). Firstly, AbE was prepared using a previously standardized methodology. The films were prepared by casting technique and cross-linked with Ca using CaCl as cross-linking agent. The physicochemical, morphological and water vapor barrier properties of the films were analyzed and the pre-clinical efficacy was investigated against the cutaneous wound model in mice. The films showed barrier properties to water vapor promising for wound healing. AbE showed physical and chemical interactions between both polymers, noticed by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and thermal analysis. The delivery of AbE in alginate/PVA films enhanced the antioxidant and wound healing properties of these polymers. Consequently, a reduction of malondialdehyde levels was observed, as well as an increase of the epidermis/dermis thickness and enhancement in collagen I deposition. Thus, these formulations are promising biomaterials for wound care and tissue repairing.
本工作旨在开发和评估基于海藻酸钠、聚乙烯醇（PVA）和负载有 Agaricus blazei Murill 水醇提取物（AbE）的 Ca 的膜的伤口愈合活性。首先，使用先前标准化的方法制备 AbE。通过浇铸技术制备膜，并使用 CaCl2 作为交联剂与 Ca 交联。分析了膜的物理化学、形态和水蒸气阻隔性能，并在小鼠皮肤伤口模型中研究了其临床前疗效。该膜对水蒸气具有阻隔性能，有望用于伤口愈合。AbE 显示出两种聚合物之间的物理和化学相互作用，傅里叶变换红外光谱、X 射线衍射、扫描电子显微镜和热分析都证实了这一点。AbE 在海藻酸钠/PVA 膜中的递送增强了这些聚合物的抗氧化和伤口愈合性能。因此，观察到丙二醛水平降低，表皮/真皮厚度增加，以及胶原蛋白 I 沉积增强。因此，这些配方是用于伤口护理和组织修复的有前途的生物材料。
