a Department of Chemical Engineering , Hanseo University , Chungcheongnam-Do , South Korea.
c Seoryeong High School , Chungcheongnam-Do , South Korea.
Artif Cells Nanomed Biotechnol. 2016 May;44(3):847-52. doi: 10.3109/21691401.2014.986676. Epub 2014 Dec 15.
The aim of this study was to develop novel biomedicated electrospun nanofibers for controlled release. Pre-formulation studies were carried out for nanofibers of sodium alginate (SA) (2 wt %)/polyvinyl alcohol (PVA) (10 wt %) composites (2/8, 3/7 and 4/6), by an electrospinning technique. The morphology and average diameter of the nanofibers were investigated by scanning electron microscopy (SEM). The optimum ratio (3/7) was used to load gatifloxacin hydrochloride (GH) (1wt %), found to form smooth fibers with uniform structures. The drug entrapment in the composite nanofibers was confirmed by SEM, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermo gravimetric analysis (TGA), differential scanning calorimetry (DSC), and swelling behavior. The drug release behavior was investigated using phosphate-buffered saline (PBS) (pH 7.4) at 37°C for 24 h. The XRD and FTIR data demonstrate that there are good interactions between PVA and SA, possibly caused by hydrogen bonds. As much as 90% of the GH was released from the electrospun fibers within 6 h of incubation. Beyond this, the release was sustained for 24 h. The thickness of nanofibers greatly influenced the initial release and rate of drug release. Moreover, GH-loaded sodium alginate/PVA composite nanofibers exhibited a useful and convenient method for electrospinning in order to control the rate and period of drug release in wound-healing applications.
本研究旨在开发用于控制释放的新型生物医学电纺纳米纤维。通过电纺技术对海藻酸钠（SA）（2wt%）/聚乙烯醇（PVA）（10wt%）复合材料（2/8、3/7 和 4/6）的纳米纤维进行了预配方研究。通过扫描电子显微镜（SEM）研究了纳米纤维的形态和平均直径。使用最佳比例（3/7）负载盐酸加替沙星（GH）（1wt%），形成了具有均匀结构的光滑纤维。通过 SEM、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、热重分析（TGA）、差示扫描量热法（DSC）和溶胀行为证实了药物在复合纳米纤维中的包封。在 37°C 的磷酸盐缓冲盐水（PBS）（pH7.4）中研究了药物释放行为，持续 24 小时。XRD 和 FTIR 数据表明，PVA 和 SA 之间存在良好的相互作用，可能是由氢键引起的。在孵育 6 小时内，多达 90%的 GH 从电纺纤维中释放出来。在此之后，释放持续了 24 小时。纳米纤维的厚度极大地影响了药物的初始释放和释放速率。此外，载有 GH 的海藻酸钠/PVA 复合纳米纤维显示出一种有用且方便的电纺方法，可控制在伤口愈合应用中药物释放的速率和周期。
