Mlicka Agnieszka, Mlicki Paweł, Niewiadomski Paweł, Zielińska Wioletta, Hałas-Wiśniewska Marta, Izdebska Magdalena
Students Research Group of Cell Biology and Ultrastructure at Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland.
Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Karłowicza 24, 85-092 Bydgoszcz, Poland.
Acta Histochem. 2023 Jan;125(1):151975. doi: 10.1016/j.acthis.2022.151975. Epub 2022 Nov 28.
The motor ability of cancer cells to cross the basement membrane contributes to their implantation in a new location. Metastasis is a significant factor that worsens the prognosis of cancer patients. Thus, reducing cell invasiveness is an important aspect of anticancer therapy, also in bladder cancer treatment.
The study material was the T24 cell line of human urinary bladder cancer. The migratory potential of the cells and the effect of the treatment with individually doses and synergistic combination of doxorubicin and metformin in the 500:1 ratio for 24 h were analyzed.
The results obtained show a compound-initiated decrease in the motor abilities of bladder cancer cells compared to controls. A decrease in the rate of colony formation was observed, as well as inhibition of migration through inserts. The visualized reorganization of the vimentin and actin networks confirms the drug-initiated limitation of the metastatic potential of T24 cells.
According to our knowledge, we are the first to show, that combination of doxorubicin and metformin also worth considering in the treatment of bladder cancer. We showed that simultaneous administration of these cytostatic enhances the antiproliferative effect of drugs, but also limits cells' migratory potential.
癌细胞穿越基底膜的运动能力有助于其在新位置的着床。转移是恶化癌症患者预后的一个重要因素。因此，降低细胞侵袭性是抗癌治疗的一个重要方面，在膀胱癌治疗中也是如此。
研究材料为人膀胱癌T24细胞系。分析了细胞的迁移潜力以及以500:1的比例单独使用阿霉素和二甲双胍剂量以及协同组合处理24小时的效果。
与对照组相比，所得结果显示复合处理引发了膀胱癌细胞运动能力的下降。观察到集落形成率降低，以及通过小室的迁移受到抑制。波形蛋白和肌动蛋白网络的可视化重组证实了药物引发的T24细胞转移潜力的限制。
据我们所知，我们首次表明，阿霉素和二甲双胍的联合用药在膀胱癌治疗中也值得考虑。我们表明，同时给予这些细胞抑制剂可增强药物的抗增殖作用，还可限制细胞的迁移潜力。
