Ebrahimi Alireza, Reza Farahpour Mohammad, Amjadi Sajed, Mohammadi Maryam, Hamishehkar Hamed
Student research committee, Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Int J Pharm. 2023 Jan 5;630:122457. doi: 10.1016/j.ijpharm.2022.122457. Epub 2022 Nov 28.
Spirulina platensis is a type of blue-green algae that contains large amounts of protein with therapeutic effects. The present study was performed to investigate the effects of encapsulated Spirulina protein hydrolysates (SPH) with nanoliposomes (NLPs) in reducing wound healing period. SPH-loaded NLPs showed the size and zeta potential of 158 nm and -48 mV, respectively; as well as a uniform non-aggregated morphology. In-vitro MTT toxicity studies on the Human Foreskin Fibroblast (HFFF-2) cell line exhibited that the hydrolyzed peptides had no toxic effect and increased cell growth. The scratch test confirmed the MTT results. For in-vivo study, 162 mice were divided into nine groups, including the mice groups treated with blank gel, blank NLPs, and those treated with 2.5, 5, and 10 % SPH and SPH-loaded NLPs. The histopathological assessment was done to investigate rate of fibroblast proliferation and epithelialization. Immunofluorescence staining for bFGF, CD31, COL1A was conducted. The results showed that the mice group treated with SPH-NLPs showed higher wound contraction, epithelization, fibroblast proliferation, and higher expressions for bFGF, CD31, COL1A compared with blanks and other groups. In conclusion, the derived and encapsulated peptides showed significant effects in accelerating wound healing via angiogenesis and collagen production.
钝顶螺旋藻是一种蓝藻，含有大量具有治疗作用的蛋白质。本研究旨在探讨纳米脂质体包裹的螺旋藻蛋白水解物（SPH）对缩短伤口愈合时间的影响。负载SPH的纳米脂质体的大小和zeta电位分别为158 nm和 -48 mV，且形态均匀无聚集。对人包皮成纤维细胞（HFFF-2）细胞系进行的体外MTT毒性研究表明，水解肽无毒性作用且能促进细胞生长。划痕试验证实了MTT结果。在体内研究中，将162只小鼠分为9组，包括用空白凝胶、空白纳米脂质体处理的小鼠组，以及用2.5%、5%和10%的SPH及负载SPH的纳米脂质体处理的小鼠组。通过组织病理学评估来研究成纤维细胞增殖和上皮形成的速率。进行了碱性成纤维细胞生长因子（bFGF）、CD31、I型胶原蛋白（COL1A）的免疫荧光染色。结果表明，与空白组和其他组相比，用SPH-纳米脂质体处理的小鼠组伤口收缩、上皮形成、成纤维细胞增殖程度更高，且bFGF、CD31、COL1A的表达也更高。总之，所制备和包裹的肽通过血管生成和胶原蛋白生成在加速伤口愈合方面显示出显著效果。
