Zheng Zhe, Hao Ruijuan, Yang Chuangye, Jiao Yu, Wang Qingheng, Huang Ronglian, Liao Yongshan, Jian Jianbo, Ming Yao, Yin Lixin, He Weiming, Wang Ziman, Li Chuyi, He Qi, Chen Kun, Deng Yuewen, Du Xiaodong
Fisheries College, Guangdong Ocean University, Zhanjiang, China.
Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, China.
Mol Ecol Resour. 2023 Apr;23(3):680-693. doi: 10.1111/1755-0998.13743. Epub 2022 Dec 13.
Biomineralization-controlled exo-/endoskeleton growth contributes to body growth and body size diversity. Molluscan shells undergo ectopic biomineralization to form the exoskeleton and biocalcified "pearl" involved in invading defence. Notably, exo-/endoskeletons have a common ancestral origin, but their regulation and body growth are largely unknown. This study employed the pearl oyster, Pinctada fucata marntensii, a widely used experimental model for biomineralization in invertebrates, to perform whole-genome resequencing of 878 individuals from wild and breeding populations. This study characterized the genetic architecture of biomineralization-controlled growth and ectopic biomineralization. The insulin-like growth factor (IGF) endocrine signal interacted with ancient single-copy transcription factors to form the regulatory network. Moreover, the "cross-phylum" regulation of key long noncoding RNA (lncRNA) in bivalves and mammals indicated the conserved genetic and epigenetic regulation in exo-/endoskeleton growth. Thyroid hormone signal and apoptosis regulation in pearl oysters affected ectopic biomineralization in pearl oyster. These findings provide insights into the mechanism underlying the evolution and regulation of biomineralization in exo-/endoskeleton animals and ectopic biomineralization.
生物矿化控制的外骨骼/内骨骼生长有助于身体生长和体型多样性。软体动物的贝壳经历异位生物矿化以形成外骨骼和参与入侵防御的生物钙化“珍珠”。值得注意的是，外骨骼/内骨骼有共同的祖先起源，但其调控和身体生长在很大程度上尚不清楚。本研究利用马氏珠母贝（Pinctada fucata marntensii），一种在无脊椎动物生物矿化研究中广泛使用的实验模型，对来自野生和养殖群体的878个个体进行了全基因组重测序。本研究对生物矿化控制的生长和异位生物矿化的遗传结构进行了表征。胰岛素样生长因子（IGF）内分泌信号与古老的单拷贝转录因子相互作用形成调控网络。此外，双壳贝类和哺乳动物中关键长链非编码RNA（lncRNA）的“跨门类”调控表明外骨骼/内骨骼生长中存在保守的遗传和表观遗传调控。珍珠贝中的甲状腺激素信号和细胞凋亡调控影响了珍珠贝的异位生物矿化。这些发现为外骨骼/内骨骼动物生物矿化的进化和调控机制以及异位生物矿化提供了见解。
